Sequences from confirmation amplicon


Confirmation sequences for line 872D03 and hit At1g71690 are shown.
Line ID 872D03
Locus At1g71690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26948525
Primers used for PCR 18jk - 8474, show primer details
   
Sequence Name 92-K113193-0022-872-D03C-18jk
GenBank Accession LN515202 [GenBank]
Sequencing primer gene specific primer, 18jk: 5'-TTGTAATAAGATCCCTCCTTCCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K113193-0022-872-D03C-18jk
TCCCAGCAAACTCTCTCCGAAATCTCCGTCACCAAGAAGGTAACAAATTAAAGTATACTA
TTTAATTATCACAAAAAAAAAGACTTTTGACATGCAAATTAAATTGTAAACTGTAGATTG
TTAATTGATATCTAAATGAAGAACTCAATTCGTCATCTAAATTCTGGAAAAATCAAATAT
GATTAGCAAATTAAATGAATCTAGTCTAATCTTTTGTACTAATGTTGCTACCGTAACATA
AGTTTAGTAGTCCGGTAATGATGTGAATTTTTAGTCTTTTAGACGTCACAAAAAATGAGC
AAATGACCGTAGTTAAATTTTATTTTACTTTTTTACTAGTAAATTGTTGTCGTATTGTAT
AAGATAAGTGTAAATGTGAATTATAGATGATACAAATTAAATTGGATCAGGAATTAGAGA
AGAAATCTCCGTGCAACTTCTTGGTATTTGGTCTTGGTCAGGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTAT
   
Sequence Name 55-K113192-0022-872-D03C-8409
GenBank Accession LN508866 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K113192-0022-872-D03C-8409
TTGAATATATCCTGACCAAGACCAAATACCAAGAAGTTGCACGGAGATTTCTTCTCTAAT
TCCTGATCCAATTTAATTTGTATCATCTATAATTCACATTTACACTTATCTTATACAATA
CGACAACAATTTACTAGTAAAAAAGTAAAATAAAATTTAACTACGGTCATTTGCTCATTT
TTTGTGACGTCTAAAAGACTAAAAATTCACATCATTACCGGACTACTAAACTTATGTTAC
GGTAGCAACATTAGTACAAAAGATTAGACTAGATTCATTTAATTTGCTAATCATATTTGA
TTTTTCCAGAATTTAGATGACGAATTGAGTTCTTCATTTAGATATCAATTAACAATCTAC
AGTTTACAATTTAATTTGCATGTCAAAAGTCTTTTTTTTTGTGATAATTAAATAGTATAC
TTTAATTTGTTACCTTCTTGGTGACGGAGATTTCGGAGAGAGTTTGCTGGGGAGTGACAT
TGGAGGAGGCATAGTGGACGAGAGCATCGGCGAGGGAAGGAGGGATCTTTATTACA

Last Updated on 06.12.2018 16:11