Sequences from confirmation amplicon


Confirmation sequences for line 872E08 and hit At4g01830 are shown.
Line ID 872E08
Locus At4g01830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:785738
Primers used for PCR 25ww - 8474, show primer details
   
Sequence Name 52-K118874-0022-872-E08C-25ww
GenBank Accession LN507971 [GenBank]
Sequencing primer gene specific primer, 25ww: 5'-GCTCTGGTTCTGACCAATAGAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K118874-0022-872-E08C-25ww
GAAACCAACACCGTTTCTATAGCTCCGATCGAACCAACGATCATCAACAACACATCGGTA
GAATCTGAGAAGGAAAACAATTTGTAGAACGGTACGGTCTTTGTCTTTTCTTCCATTTCT
ATCTTTTTTGTTTTCCTTTTTTCTAACTACAAACATGATTGATTCATTTCTTATGTCTCT
TTCATTTGATATTTTCTTAAAATGATTCTATTTAATGTTTAATGGCCCAATTGAAGTGAT
CTATATTATTTTAAGAACAAAACTGATCATTAGTTATAAAATATGAAAATATCGTCAGTC
TTGTGTCGTCGTGTATGCCACCATTATTCCTTATTGTATTATGTTAATAGTGTGTTTAAG
CATTGAACAAGCCGGAGTTCTCGTCCCTTTTTAGATTATCACATGTGATATGTGAAAAAT
GACAGTTGCGTAGCTAAAAAGCAATGACTAATAACAATAAATGTTTGCGTCAATTAGAAG
CATTGATGTGGAGTTGAACGAGCGAGGCATAAACTCCACCTTCGATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
   
Sequence Name 12-K118873-0022-872-E08C-8409
GenBank Accession LN491809 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K118873-0022-872-E08C-8409
TCGAAGGTGGAGTTTATGCCTCGCTCGTTCAACTCCCACTCTTGCTTCTAATTGACGCAA
ACATTTATTGTTATTAGTCATTGCTTTTTAGCTACGCAACTGTCATTTTTCACATATCAC
ATGTGATAATCTAAAAAGGGACGAGAACTCCGGCTTGTTCAATGCTTAAACACACTATTA
ACATAATACAATAAGGAATAATGGTGGCATACACGACGACACAAGACTGACGATATTTTC
ATATTTTATAACTAATGATCAGTTTTGTTCTTAAAATAATATAGATCACTTCAATTGGGC
CATTAAACATTAAATAGAATCATTTTAAGAAAATATCAAATGAAAGAGACATAAGAAATG
AATCAATCATGTTTGTAGTTAGAAAAAAGGAAAACAAAAAAGATAGAAATGGAAGAAAAG
ACAAAGACCGTACCGTTCTACAAATTGTTTTCCTTCTCAGATTCTACCGATGTGTTGTTG
ATGATCGTTGGTTCGATCGGAGCTATAGGAAACGGTGTTGGTTTTCCTTTAATGACATTA
TTGTTTGGTGATCTCATTGATTCTATTGGTCAAAAACCAGAG

Last Updated on 06.12.2018 16:11