Sequences from confirmation amplicon


Confirmation sequences for line 873C10 and hit At5g25310 are shown.
Line ID 873C10
Locus At5g25310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8785200
Primers used for PCR 36jx - 8474, show primer details
   
Sequence Name 26-K113982-0022-873-C10C-36jx
GenBank Accession LN498021 [GenBank]
Sequencing primer gene specific primer, 36jx: 5'-AAAACCAGTGGTGTGTACATGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K113982-0022-873-C10C-36jx
GATTCGTTAGCGTCATTAGAAGAATCTACTAAAATCCACGCTAATTTAGCCGTGCGCAGT
GACTTTAAAGCTAAGTATTTCAAAAAAGTAATTGCATGAAACTTAGAGCTTACCGATATA
AAGCTGATGGATTACGATAAATTTCTGAGTTTGGAAGATCAGATTTGAACAATGTAGTGT
TAACATTCGAAGAAGCTTCTAAAATCGATGCTCTTGCTTTCGCTAATCCTTGTTCCACCA
AATTTCTCCGGTTTAGTTTTTCCGGTTTACTCTGTAACTGATTCAACAAACAATTTCCGA
TGATGATTAGCCAAAAAATGCAGGATCATCAGATTTTGGACGATGCTNATGATGATGATG
ATGATGATGATGATGATGATGGTGGTAATGATGGTGATGATGCTGATGANAAATTCTCAT
TTTCAAATTTTGAAGATNAANGATNATNAGAGATAATTTTANANAGATNTATTNAATTGT
AAATGGNTTCATGTNNGGGAAATNTACNTGGATTAGNAATGAGTATGATGGTNAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTT
   
Sequence Name 10-K113981-0022-873-C10C-8409
GenBank Accession LN490746 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K113981-0022-873-C10C-8409
CATTTCATTGAATATATCTGTGTAAATTATCTCTAATCATCGTTGATCTTCAAAATTTGA
AACTGAGAATTTCTCATCAGCATCATCACCATCATTACCACCATCATCATCATCATCATC
ATCATCATCATCATTAGCATCGTCCAAAATCTGATGATCCTGCATTTTTTGGCTAATCAT
CATCGGAAATTGTTTGTTGAATCAGTTACAGAGTAAACCGGAAAAACTAAACCGGAGAAA
TTTGGTGGAACAAGGATTAGCGAAAGCAAGAGCATCGATTTTAGAAGCTTCTTCGAATGT
TAACACTACATTGTTCAAATCTGATCTTCCAAACTCAGAAATTTATCGTAATCCATCAGC
TTTATATCGGTAAGCTCTAAGTTTCATGCAATTACTTTTTTGAAATACTTAGCTTTAAAG
TCACTGCGCACGGCTAAATTAGCGTGGATTTTAGTAGATTCTTCTAATTGACGCTNACGA
ATCCATGAAATTGAACCAATTAAACTCAATTTGATACTTTTTTCATGTAC

Last Updated on 06.12.2018 16:11