Sequences from confirmation amplicon


Confirmation sequences for line 873G01 and hit At4g14280 are shown.
Line ID 873G01
Locus At4g14280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8223148
Primers used for PCR 39wx - 8474, show primer details
   
Sequence Name 32-K119249-0022-873-G01C-39wx
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 39wx: 5'-GCTGTTCTTGAGAAGGTTTTTGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K119249-0022-873-G01C-39wx
TTTCTTGAGTTTTAAATTGTGTTCTTAAATCTTAATCACAGATTGCTTCAGGGTTGGGAT
CTTTAGGTTTCGTGTGGGCCACGGTTGTTCTTCTTGGTGGATTCGCTGGAAGTTTAGAGA
TAACAGATTTCTGGTTTGTTACTGTGATTCTTGTTATTGAAGGAGCTAGGCTTTTCAGTA
GAAGCCACGAGCTTGAACTGCAGCATCAGTCTAAGTACACAATCTCTGGAATCAACATCT
TCCGGTTCCTTGTCAAACAAATTAACCAAATATTTCACCAGGTGGCTCACATTGCCGGAG
ACGACAATAGACCATCGGTTCGAGAAACGAGAACCGTACAAAGGAATTCGGGACACATCA
CGCGGACAAGGACATGGAAAAGCTCAGATGTTCCTATGTTGCCTTACACAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
G
   
Sequence Name 16-K119248-0022-873-G01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K119248-0022-873-G01C-8409
ATTTCATTGAATATATCCTGTGTAAGGCAACATAGGAACATCTGAGCTTTTCCATGTCCT
TGTCCGCGTGATGTGTCCCGAATTCCTTTGTACGGTTCTCGTTTCTCGAACCGATGGTCT
ATTGTCGTCTCCGGCAATGTGAGCCACCTGGTGAAATATTTGGTTAATTTGTTTGACAAG
GAACCGGAAGATGTTGATTCCAGAGATTGTGTACTTAGACTGATGCTGCAGTTCAAGCTC
GTGGCTTCTACTGAAAAGCCTAGCTCCTTCAATAACAAGAATCACAGTAACAAACCAGAA
ATCTGTTATCTCTAAACTTCCAGCGAATCCACCAAGAAGAACAACCGTGGCCCACACGAA
ACCTAAAGATCCCAACCCTGAAGCAATCTGTGATTAAGATTTAAGAACACAATTTAAAAC
TCAAGAAAAGGACTCCTAAATAATGAACTAGAATGTTGGAATCTCAAAAACCTTCCAAGG
GAACAG

Last Updated on 06.12.2018 16:11