Sequences from confirmation amplicon


Confirmation sequences for line 873G04 and hit At3g56060 are shown.
Line ID 873G04
Locus At3g56060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20804990
Primers used for PCR KQ89 - 8474, show primer details
   
Sequence Name 26-K101500-0022-873-G04C-8409
GenBank Accession LN497857 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K101500-0022-873-G04C-8409
GCCATTTACATTGATCACGTGTAAGCTACGTCTCTCTGACTCTCGCTTTGTTTTAGGTTT
TTCAGGAACGTTCCTCTCTCTTCTTTCATACCTCTCGTTATGGAAAAATAAAATAAATTA
TAAGACATGTATTCAATTATCAGTCGTCATTACCTTTAATAGTGAAGAGGATCTTATGGA
CAGAAGCATGCAAGTAGACAACTATCATGTTCGGATTAGCATACTCCAACAAATTCGCCG
CCGTGTGTCTATGACCATCCCGGTCAAATATCGTACCACCAAATTTAGTCCCAACGATAT
GTTCGTACGTGAAACCATTGTATGGAGTCACACCAGCTTCCAAAAGCCCATCTCGAAACG
CTGATTGCCATTTGTTAACCGGTGGTTCAAAAACCACTTTCTTCTCGACCCACTCGTAAG
CCGCTTCGACCTCGTCTCTCTCCCATCCTGCTTCCGCCACGAAATCTTCTTCCGCACGTG
AGTAGAATCCTGCGTTTA
   
Sequence Name 26-K101504-0022-873-G04C-KQ89
GenBank Accession LN497858 [GenBank]
Sequencing primer gene specific primer, KQ89: 5'-CACAGTCATAAACGCAGGATTCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K101504-0022-873-G04C-KQ89
GGCGGAGCAGGATGGGAGAGAACGAGGTCGAAGCGGCTTACGAGTGGGTCGAGAAGAAAG
TGGTTTTTGAACCACCGGTTAACAAATGGCAATCAGCGTTTCGAGATGGGCTTTTGGAAG
CTGGTGTGACTCCATACAATGGTTTCACGTACGAACATATCGTTGGGACTAAATTTGGTG
GTACGATATTTGACCGGGATGGTCATAGACACACGGCGGCGAATTTGTTGGAGTATGCTA
ATCCGAACATGATAGTTGTCTACTTGCATGCTTCTGTCCATAAGATCCTCTTCACTATTA
AAGGTAATGACGACTGATAATTGAATACATGTCTTATAATTTATTTTATTTTTCCATAAC
GAGAGGTATGAAAGAAGAGAGAGGAACGTTCCTGAAAAACCTAAAACAAAGCGAGAGTCA
GAGAGACGTAGCTNACACGTGATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11