Sequences from confirmation amplicon


Confirmation sequences for line 874C07 and hit At1g29550 are shown.
Line ID 874C07
Locus At1g29550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10331244
Primers used for PCR MJ58 - 8474, show primer details
   
Sequence Name 38-K029914-022-874-C07C-8409
GenBank Accession LN502868 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K029914-022-874-C07C-8409
TTCATCCTCCAACCAGAGGGTTTCGGGGGCGGATCTTTACTGGCTTCAAAGATAAGATTG
AACCAAAATGGGAAGATCCTATCTGTGCTAATGGAGGAAAATGGAGTATGATGTTCCCTA
AAGCTACACTAGAATGCAATTGGCTTAACACGGTATTGTAACTTTTCAGGACTTCATTGT
AGTTTTTCCCTTGGCTATTTTGCTTGTTTGATGCATCTTTGTGATGTTTCTTGTCTCCTT
TTTTTAGTTACTTGCGTTGGTTGGTGAGCAATTTGACCAAGGAGATGAGATCTGTGGAGC
AGTTTTGAACTTCAGAGCGAGAGGGGACAGGATCTCTCTATGGACAAAGAACGCTGCAAA
TGAGGAGGCTCAGGTAAAACATGGATGCCTTATATTAGATATTTATCTGGTAACCAAAAA
GGCTTGTTCTTGAGGG
   
Sequence Name 38-K029914-022-874-C07C-MJ58
GenBank Accession LN502869 [GenBank]
Sequencing primer gene specific primer, MJ58: 5'-ATTCACCAAATACTTGTGTCTCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K029914-022-874-C07C-MJ58
TCTAATATAAGGCATCCATGTTTTACCTGAGCCTCCTCATTTGCAGCGTTCTTTGTCCAT
AGAGAGATCCTGTCCCCTCTCGCTCTGAAGTTCAAAACTGCTCCACAGATCTCATCTCCT
TGGTCAAATTGCTCACCAACCAACGCAAGTAACTAAAAAAAGGAGACAAGAAACATCACA
AAGATGCATCAAACAAGCAAAATAGCCAAGGGAAAAACTACAATGAAGTCCTGAAAAGTT
ACAATACCGTGTTAAGCCAATTGCATTCTAGTGTAGCTTTAGGGAACATCATACTCCATT
TTCCTCCATTAGCACAGATAGGATCTTCCCATTTTGGTTCAATCTTATCTTTGAAGCAGT
AAAGATCCGCCCCCGAAACCCACTTGGTTGGAGGATGAATGTTATTGTAGAGACTGCATC
AAGAAATCCAACATACATAGCTTGCCGAGTAAATGGCTATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT

Last Updated on 06.12.2018 16:11