Sequences from confirmation amplicon


Confirmation sequences for line 875F11 and hit At5g27670 are shown.
Line ID 875F11
Locus At5g27670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9792933
Primers used for PCR JE08 - 8474, show primer details
   
Sequence Name 83-K035351-022-875-F11C-JE08
GenBank Accession LN514266 [GenBank]
Sequencing primer gene specific primer, JE08: 5'-AATCCAAGATTGAAACCAAACAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K035351-022-875-F11C-JE8
ATTCTAAACCCCACCGGAAAACCAAAACCCCTTTTCCAGAATCTCGTAGATGAACCAAGA
ACATAAATTCTAACGGGCCTATCAACTAAGCCCAAACAAAGCCCAATTTGAACAATCATT
TTCTTAATTAATGTCATGTTTTTCGTGATTATTATCTACTTTAATTAATGTCATTAACTG
TACTGAATCTTTCCATGTGTATACCAAAACCATTTCAATTAATCCACCTAAATTGTTTAA
ATCAACAAGTACGAAACCCAGAAATTAATTAGACAGATGAAGTTACTAATCCCAGAACTT
AATTATTTTCCATTACAAATTGCGTTAACAAACATAATCATCTTCCCATGGTTTTTACAT
AAGACAAACCAAAACAAAGAGAAGAAGATTTCAGATACCTACACAAATCCTCTAAAACAA
AAAACAAGTTGTTTCTTCTTCTTCTTCTTCTTCATCTAAGATCCGAATATAACCAAAAAG
CAACATCGATACTATTCTTTATCAAGCCTTCTTAGGAGATTTGGTAGCAGAAGCTTTCTC
CGCTTGAGAAGAAGAAGCTGTTGATTTCTTAGGAAGAAGAACTGGATTAATGTTTGGAAG
AACACCACCACTAGCAATAGTAACTCCAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATT
   
Sequence Name 35-K035351-022-875-F11C-8409
GenBank Accession LN501735 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K035351-022-875-F11C-8409
TCCGGACTGAAGCCTTTAAATTGAAATATCCTGGAGTTACTATTGCTAGTGGTGGTGTTC
TTCCAACATTAATCCAGTTCTTCTTCCTAAGAAATCAACAGCTTCTTCTTCTCAAGCGGA
GAAAGCTTCTGCTACCAAATCTCCTAAGAAGGCTTGATAAAGAATAGTATCGATGTTGCT
TTTTGGTTATATTCGGATCTTAGATGAAGAAGAAGAAGAAGAAGAAACAACTTGTTTTTT
GTTTTAGAGGATTTGTGTAGGTATCTGAAATCTTCTTCTCTTTGTTTTGGTTTGTCTTAT
GTAAAAACCATGGGAAGATGATTATGTTTGTTAACGCAATTTGTAATGGAAAATAATTAA
GTTCTGGGATTAGTAACTTCATCTGTCTAATTAATTTCTGGGTTTCGTACTTGTTGATTT
AAACAATTTAGGTGGATTAATTGAAATGGTTTTGGTATACACATGGAAAGATTCAGTACA
GTTAATGACATTAATTAAAGTAGATAATAATCACGAAAAACATGACATTAATTAAGAAAA
TGATTGTTCAAATTGGGCTTTGTTTGGGCTTAGTTGATAGGCCCGTTAGAATTTATGTTC
TTGGTTCATCTACGAGATTCTGGAAAAGGGGTTTTGGTTTTCCGGTGGGGTTTAGAATTT
AAACAAGACGCGATTTCGAATTTCGTTCTTGTAGAATCAAATTGTTTGGTTTCANTCTTG
GA

Last Updated on 06.12.2018 16:11