Sequences from confirmation amplicon


Confirmation sequences for line 876D09 and hit At3g55770 are shown.
Line ID 876D09
Locus At3g55770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20704177
Primers used for PCR CL16 - 8474, show primer details
   
Sequence Name 07-K028743-022-876-D09C-8409
GenBank Accession LN489131 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K028743-022-876-D09C-8409
AAAGTTCTTGTTGAAAACTCCCACTTCTCCTTAAATGAGCTCGCTTCAAAACGTGGCTTA
ACAGTACAAAACACCTTCCATTGATGAGTAAACTACTCAGCCGTAAAAGACATTTACTTT
ATCAAGAGCTGCTTAAGGTTACAATAGACATTCAATTCATCATTTAAGCAAGAAGATGGG
TAATTCGTAAACTCATTCCTCTAATTAAAGCAGCATCGTTAACATGAATACAAAGATGAG
AGAATCAGACCTGAAGCCTGCTTTTGCAGTGAGTGCATTTGAAGCAAGACTTGTGATATC
CAACTCCATCAGCAGAGAGAAGCTCAACAGCATAAACAGTCTTCTCACAAGCCTTGCATT
TCTGTTGAGTTCCTGTAAAAGACA
   
Sequence Name 07-K028743-022-876-D09C-CL16
GenBank Accession LN489132 [GenBank]
Sequencing primer gene specific primer, CL16: 5'-AAGGCTTTGAGGATGTCTTTTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K028743-022-876-D09C-CL16
CACAAGTTCTTGCTTCAAATGCACTCACTGCAAAAGCAGGCTTCAGGTCTGATTCTCTCA
TCTTTGTATTCATGTTAACGATGCTGCTTTAATTAGAGGAATGAGTTTACGAATTACCCA
TCTTCTTGCTTAAATGATGAATTGAATGTCTATTGTAACCATAAGCAGCTCTTGATAAAG
TAAATGTCTTTTTACAGCTGAGTAGTTACTCATCAATGGAAGGTGTTTTGTACTGTAAGC
CTCATTTTGAGCAGCTCTTTAAGGAGAGTGGTAGTTTCAACAAGAACTTTCAGTCACGTA
TGTAGTTTTGTTTCTGCTTCGATTCATGTTTTTTTTCCGCTTGCCCTTCTTTTGGTTATT
CATGTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11