Sequences from confirmation amplicon


Confirmation sequences for line 876F11 and hit At2g15370 are shown.
Line ID 876F11
Locus At2g15370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:6698376
Primers used for PCR 28xs - 8474, show primer details
   
Sequence Name 52-K119546-0022-876-F11C-28xs
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 28xs: 5'-GGGCTACAATAAGGAATATCCTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K119546-0022-876-F11C-28xs
TCACTCGACTTCATCCCGCCGCATCTATCATGCATAACCTTCATGATTCAAGGGATAGTG
ATAAGCTGTTTGTATGCCAAAAGGATCAAAGTTTGATTGACAAAGTCCCATGGTTGATTG
TTCAAGCCAATGTTTACTTTGTTCCATCGTTATGGTTTAATCCAACTTTCCAAACCGAAC
TAGTTAAGCTGTTCCCGCAGAAAGAAACCGTCTTTCACCACTTGGCTCGGTATCTTTTTC
ACCCTACAAATGAAGTTTGGGATATGGTCACTGACTACTACCACGCTCATTTGTCGAAAG
CCGACGAGAGACTCGGGATTCAAATAAGGGTTTTCGGCAAACCTGATGGACGTTTCAAAC
ATGTCATTGACCAGGTCATATCATGTACACAAAGAGAGAAACTGTTACCTGAATTTGCTA
CACCAGAGGAATCAAAAGTCAATATATCAAAAACCCCGAAACTCAAATCTGTTCTTGTCG
CATCTCTCTATCCAGAGTTCTCTGGCAACTTAACTAACATGTTTTCAAAGCGACCAAGTT
CAACAGGAGAAATTGTTGAAGTTTATCAACCAAGTGGAGAGAGAGTTCAGCAAACAGACA
AGAAAAGTCACGACCAAAAGGCGCTTGCTGAGATGTATCTNTTGAGCGCAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
   
Sequence Name 20-K119545-0022-876-F11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K119545-0022-876-F11C-8409
ATTTCATTGAATATATCCTGCGCTCAAAGATACATCTCAGCAAGCGCCTTTTGGTCGTGA
CTTTTCTTGTCTGTTTGCTGAACTCTCTCTCCACTTGGTTGATAAACTTCAACAATTTCT
CCTGTTGAACTTGGTCGCTTTGAAAACATGTTAGTTAAGTTGCCAGAGAACTCTGGATAG
AGAGATGCGACAAGAACAGATTTGAGTTTCGGGGTTTTTGATATATTGACTTTTGATTCC
TCTGGTGTAGCAAATTCAGGTAACAGTTTCTCTCTTTGTGTACATGATATGACCTGGTCA
ATGACATGTTTGAAACGTCCATCAGGTTTGCCGAAAACCCTTATTTGAATCCCGAGTCTC
TCGTCGGCTTTCGACAAATGAGCGTGGTAGTAGTCAGTGACCATATCCCAAACTTCATTT
GTAGGGTGAAAAAGATACCGAGCCAAGTGGTGAAAGACGGTTTCTTTCTGCGGGAACAGC
TTAACTAGTTCGGTTTGGAAAGTTGGATTAAACCATAACGATGGAACAAAGTAAACATTG
GCTTGAACAATCAACCATGGGACTTTGTCAATCAAACTTTGATCCTTTTGGCATACAAAC
AGCTTATCACTATCCCTTGAATCATGAAGGTTATGCATGTATAGATGCGGCGGGATTGAA
GTCGAGTTGATGGAATGTTTTTCAGACATTGTTCCGTAACAACGAGGATATTCCTTATTG
T

Last Updated on 06.12.2018 16:11