Sequences from confirmation amplicon


Confirmation sequences for line 876H04 and hit At5g51560 are shown.
Line ID 876H04
Locus At5g51560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20946577
Primers used for PCR EU89 - 8474, show primer details
   
Sequence Name 40-K103934w-0022-876-H04C-EU89
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, EU89: 5'-TGAGAAACACTTCACAAAATACGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K103934w-0022-876-H04C-EU89
TTAGATATATAGTGATCAGTTTTCAACCAGGAGGTACATTGCCAGTGAGGGAGTTGTTGC
GGATGTCGAGAACTCGAAGCAGAGGAGGGCTAGCTAACTTGCCTGGTACAGAACCAAACA
AATGATTGTAGCTCAAATCTAGCCGCTCTAGAGCACTTAAATCTCCTAAACTCGCAGGTA
TAGCTCCAGTGAGTTTGTTAGATTGAAGAGCAAGAACACTCAGCTTCCTCAGTGAACTAA
GCTCCCTAGGAATGCTTCCAGTCAAATTGTTGTAACAGAGCTGCAAAACTACACAACAAC
ATAGACCAAAAAAGGAATGAAGCTTAGCTTTGACTTACATTAACTTAGAACTAATTCTAG
ATTTAGAAACCTGGTCCTGCTAATTAAAGCTCAAATATAAAGCCTTTTGAAAGTAAGTGA
ATCAATTATCTAAATAAACAAAATTAATCAAGAGAAGTACAAGTTATCTTCAATTCAAGT
CCATATTTTGGGACCAGTAAGCCATTTATGGTTCCAATTAAAAGTTTGACATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
CGTTAT
   
Sequence Name 51-K103935w-0022-876-H04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K103935w-0022-876-H04C-8409
CCATTTACATTGAATATGTCAAACTTTTAATTGGAACCATAAATGGCTTACTGGTCCCAA
AATATGGACTTGAATTGAAGATAACTTGTACTTCTCTTGATTAATTTTGTTTATTTAGAT
AATTGATTCACTTACTTTCAAAAGGCTTTATATTTGAGCTTTAATTAGCAGGACCAGGTT
TCTAAATCTAGAATTAGTTCTAAGTTAATGTAAGTCAAAGCTAAGCTTCATTCCTTTTTT
GGTCTATGTTGTTGTGTAGTTTTGCAGCTCTGTTACAACAATTTGACTGGAAGCATTCCT
AGGGAGCTTAGTTCACTGAGGAAGCTGAGTGTTCTTGCTCTTCAATCTAACAAACTCACT
GGAGCTATACCTGCGAGTTTAGGAGATTTAAGTGCTCTAGAGCGGCTAGATTTGAGCTAC
AATCATTTGTTTGGTTCTGTACCAGGCAAGTTAGCTAGCCCTCCTCTGCTTCGAGTTCTC
GACATCCGCAACAACTCCCTCACTGGCAATGTACCTCCTGGTTAGAAAACTGATTCACTA
TATGATCTTTGAAACTAACTTTGCGTATTTTGTGAAGTGTTTCTC
Primers used for PCR CN31 - 8474, show primer details
   
Sequence Name 31-K118466-0022-876-H04C-CN31
GenBank Accession LN500345 [GenBank]
Sequencing primer gene specific primer, CN31: 5'-GGGAAAGGCTTATCTGGTAAAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K118466-0022-876-H04C-CN31
AGATATTCCTAGAGAACTTGGTAACTTGTCGGAACTTACGGATCTTTATCTCAATGTTAA
TAATCTCTCCGGTGAGATTCCTTCAAACATTGGGAAGATGCAAGGCTTGCAAGGTTAGTT
CTTTTTTACTTTAATTTTAATCTTAATCATTGATTAAACTTAAAACCTAAATTTAGCATT
GTTAGATCTTTGGTTTCTGCAAAATTCTTGTCCCAAGATTAATCAATGTTCTGTGGAGAT
TTGAAACCTAGAAATCTGAGCTCCTTGTCTTAGGGGTTTTAGTTACTTCATTACTATCTC
TGGTAGTTGAAATTATTTGAATTAGATGATTTTGGAATTTAATGCTTTAATGATCTGTCT
TTAAAACGTCCTTTTCATGTCTTTTTGTTAGGATTTTTCTTTTTGTAATTTTCCTGTAAG
GAAGAATAGTCAAGAACTTTTTAAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATT
   
Sequence Name 04-K118465-0022-876-H04C-8409
GenBank Accession LN488116 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K118465-0022-876-H04C-8409
CATTTACTTAAAAAGTTCTTGACTATTCTTCCTTACAGGAAAATTACAAAAAGAAAAATC
CTAACAAAAAGACATGAAAAGGACGTTTTAAAGACAGATCATTAAAGCATTAAATTCCAA
AATCATCTAATTCAAATAATTTCAACTACCAGAGATAGTAATGAAGTAACTAAAACCCCT
AAGACAAGGAGCTCAGATTTCTAGGTTTCAAATCTCCACAGAACATTGATTAATCTTGGG
ACAAGAATTTTGCAGAAACCAAAGATCTAACAATGCTAAATTTAGGTTTTAAGTTTAATC
AATGATTAAGATTAAAATTAAAGTAAAAAAGAACTAACCTTGCAAGCCTTGCATCTTCCC
AATGTTTGAAGGAATCTCACCGGAGAGATTATTAACATTGAGATAAAGATCCGTAAGTTC
CGACAAGTTACCAAGTTCTCTAGGAATATCTCCAACAAGAGCATTGTAGTGCAAGAACAA
ACCCGTCAAATGTTTAAGCTTTCCAATGTTCGGAGAGATTTTACCAGATAGCCCCTTTCC

Last Updated on 06.12.2018 16:11