Sequences from confirmation amplicon


Confirmation sequences for line 877A06 and hit At5g26910 are shown.
Line ID 877A06
Locus At5g26910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9466607
Primers used for PCR 32fr - 8474, show primer details
   
Sequence Name 46-K111470-0022-877-A06C-32fr
GenBank Accession LN505898 [GenBank]
Sequencing primer gene specific primer, 32fr: 5'-AAGTTAGCAGCATTAGCACTGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K111470-0022-877-A06C-32fr
CAGGGTTTCTCGATTGCAGACAGACCGCAGAATATGGTTCGTTTTCTTAATACTTCACAA
AGTTTTATATTTTTCATTCTCCCACGGTTTTAATTTATGAATACTCCACTGAAACTCAAC
ATTCATCCTGCAAAAACTTGAATCCTTAAGATACATGTAATGTGCCAAAACGCTGTTTGC
ACTCTATTTGTACAAGGCGTAGATCTAATGGCTTAAACAGAAGCTTGATTTGCAACTACT
TTTATCCTGTCTAACGCTTTGGGCTTTACAGGGACAATACAGTCCTCATCAGATCAAGAA
CTCACCTGGGTTTCATTAAATGAGTCGCACCAAGCACAAGACGAGTCCGAGCTCTCAGAA
TCTGTGGTAACACTGAGTTACTCAGAAGCCGAAGAAAGGCTCGACTGGGAGTTCGAGTAC
ATATCCGAGATCCTCGGGTCTGACCAACTGATGGTGAAAGAATATGCTTTGGGGATGGCC
ACTGACGTATTACCTGCGAGCCTCTTTGATGAAATGGAAGGCCGAGGAAATCTACATGAA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCACAATTA
   
Sequence Name 30-K111469-0022-877-A06C-8409
GenBank Accession LN499777 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K111469-0022-877-A06C-8409
CTCATTTCTCAAGAGGCTCGCAGGTAATACGTCAGTGGCCATCCCCAAAGCATATTCTTT
CACCATCAGTTGGTCAGACCCGAGGATCTCGGATATGTACTCGAACTCCCAGTCGAGCCT
TTCTTCGGCTTCTGAGTAACTCAGTGTTACCACAGATTCTGAGAGCTCGGACTCGTCTTG
TGCTTGGTGCGACTCATTTAATGAAACCCAGGTGAGTTCTTGATCTGATGAGGACTGTAT
TGTCCCTGTAAAGCCCAAAGCGTTAGACAGGATAAAAGTAGTTGCAAATCAAGCTTCTGT
TTAAGCCATTAGATCTACGCCTTGTACAAATAGAGTGCAAACAGCGTTTTGGCACATTAC
ATGTATCTTAAGGATTCAAGTTTTTGCAGGATGAATGTTGAGTTTCAGTGGAGTATTCAT
AAATTAAAACCGTGGGAGAATGAAAAATATAAAACTTTGTGAAGTATTAAGAAAACGAAC
CATATTCTGCGGTCTGTCTGCAATCTGAGAAACCCTTGCTGCAAGAGCTTCTTAGATCAT
CTGCTTCCGTCACAGTGCTAATGCTGCTAACT

Last Updated on 06.12.2018 16:11