Sequences from confirmation amplicon


Confirmation sequences for line 877C07 and hit At5g65020 are shown.
Line ID 877C07
Locus At5g65020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25975132
Primers used for PCR JD17 - 8474, show primer details
   
Sequence Name 34-K103233-0022-877-C07C-8409
GenBank Accession LN501377 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K103233-0022-877-C07C-8409
CACCTCACCACTACAACACGAGTATGGAAACGCCATTAACAAGGTTAGCCTAACCACCAA
CTGCTTTTTCATTTGATGTTACAAAATCTTGTTCGGAAAGCTTATTATATGAAGTGAAAT
TTTTGCAGAACTTGAAGGAAGAGTCGGACGACAATGACTACATGAAACTACTAAGAGCTG
TAATCACATGTTTGACATACCCTGAGAAGCATTTTGAGAAGGTTCTTCGTCTATCAATCA
ACAAAATGGGAACAGACGAATGGGGACTAACCCGAGTCGTGACTACACGAACTGAAGTTG
ACATGGAACGCATCAAAGAGGAATATCAGCGAAGAAACAGCATTCCTTTGGACCGTGCTA
TCGCCAAAGACACTTCTGGTGACTATGAGGACATGCTTGTTGCTCTTCTCGGACATGGCG
ATGCTTGAAACTGTTTCAACTTTCGAGTTCCTCCTTTCTCTTACTGCAA
   
Sequence Name 82-K103234-0022-877-C07C-JD17
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, JD17: 5'-TGCAGTAAGAGAAAGGAGGAACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K103234-0022-877-C07C-JD17
AGTGTTGCACCAAGCAATCTACATGGATCAGCACTGACTATGATGGTCAGTAAGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGA
AAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGT
CCAAATGGG

Last Updated on 06.12.2018 16:11