Sequences from confirmation amplicon


Confirmation sequences for line 877F12 and hit At3g19480 are shown.
Line ID 877F12
Locus At3g19480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6752889
Primers used for PCR 20be - 8474, show primer details
   
Sequence Name 41-K107369-0022-877-F12C-20be
GenBank Accession LN504098 [GenBank]
Sequencing primer gene specific primer, 20be: 5'-TGGTGCATAAGGATCATGTGTAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K107369-0022-877-F12C-20be
CACCTCTGATCAACTTTACCAAAACCAAGAACAGCAAGAGTCTTCCCCACAAGTGAAACA
CCTACATACTTGTTTCTCGTCCATTTGCCTACAAACAACCACATTTCTACATTTCACATC
GCAATTTCTCAAATCGCTATTCAAATTCAACAACCGTTTAAAACACTCACCAGCTTTGAT
AGAAGCATCAGCTTGAGCAATGTTTCTAGCCATGGCGGTCAAAAGCGCAATTCCGTGTTC
AGCAGCAGCCACAGTGTTCGCAGTCGGAGCATTAACAACCAAACACCCATACTCAGTCGC
CGCCGCCAAATCGACGTTATCAATCCCAACACCAGCGCGTCCAACAACCTTAAGCCTCCC
ACGCGACGATTCAAACACATCTCTACATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCACGNTTATTA
   
Sequence Name 57-K107370-0022-877-F12C-8409
GenBank Accession LN509437 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K107370-0022-877-F12C-8409
AGATGTGTTTGATCGTCGCGTGGGAGGCTTAAGGTTGTTGGACGCGCTGGTGTTGGGATT
GATAACGTCGATTTGGCGGCGGCGACTGAGTATGGGTGTTTGGTTGTTAATGCTCCGACT
GCGAACACTGTGGCTGCTGCTGAACACGGAATTGCGCTTTTGACCGCCATGGCTAGAAAC
ATTGCTCAAGCTGATGCTTCTATCAAAGCTGGTGAGTGTTTTAAACGGTTGTTGAATTTG
AATAGCGATTTGAGAAATTGCGATGTGAAATGTAGAAATGTGGTTGTTTGTAGGCAAATG
GACGAGAAACAAGTATGTAGGTGTTTCACTTGTGGGGAAGACTCTTGCTGTTCTTGGTTT
TGGTAAAGTTGGATCAGAGGTTGCTAGACGAGCCAGGGGACTTGGTATGCATGTCATTAC
ACATGATCCTTATGCACC

Last Updated on 06.12.2018 16:11