Sequences from confirmation amplicon


Confirmation sequences for line 879A11 and hit At2g46880 are shown.
Line ID 879A11
Locus At2g46880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19266256
Primers used for PCR 27ey - 8474, show primer details
   
Sequence Name 58-K111119-0022-879-A11C-27ey
GenBank Accession LN509737 [GenBank]
Sequencing primer gene specific primer, 27ey: 5'-AGATTGTAATTACCAAAACCGTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K111119-0022-879-A11C-27ey
GGGTTACTTGAGACACGAGTTTGGGAGTTTCATGATGTATTTCATCATTGTTTCTCTAGT
CATGTCGGATTCTTGGTCGTGATTCCCCAAAATAGCCACCCATGGAATCCCCGATTCGAT
CGCAGGAGCAAATGCCATGTCCATCGATTTTGCCACGTCACTTGTCTCGCACAATCCATA
TACATTGTCTCCTGCAATATTGTATGTGGTGTTTTAGGTTTTTTTTTTCACGCATAAACA
TTTTTTATACATACACTAAGTTAACTAAAATATTTGTATAGATAACAAAACACAATTATG
GTATAAAATTTTGTATTATTTGAAGAATTTGGTACCAAAGAAAACAATAAGATCGGGCTT
CTCGGAAGCGATAGTTCGCTGTAAAAAGGAGGTAGTATTGAGATCGGAGCAGTAAGGAAA
TTCTGCAGGTGAAACGTCTGAGCATTGCGTCTCTTTGCCAAAACCGTAGTGCATATCAGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AAATTTC
   
Sequence Name 42-K111118-0022-879-A11C-8409
GenBank Accession LN504499 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K111118-0022-879-A11C-8409
ATCCTGATATGCACTACGGGTTTTGGCAAAGAGACGCAATGCTCAGACGTTTCACCTGCA
GAATTTCCTTACTGCTCCGATCTCAATACTACCTCCTTTTTACAGCGAACTATCGCTTCC
GAGAAGCCCGATCTCATCGTCTTCTCTGGTACCAAATTCTTCAAATAATACAAAATTTTA
TACAATAATTGTGTTTTGTTATCTATACAAATATTTTAGTTAACTTAGTGTATGTATAAA
AAATGTTTATGCGTGAAAAAAAAAACCTAAAACACCACATACAATATTGCAGGAGACAAT
GTATATGGATTGTGCGAGACAAGTGACGTGGCAAAATCGATGGACATGGCATTTGCTCCT
GCGATCGAATCGGGGATTCCATGGGTGGCTATTTTGGGGAATCACGACCAAGAATCCGAC
ATGACTAGAGAAACAATGATGAAATACATCATGAAACTCCCAAACTCGTTGTCTCAAGTA
AACCCTCCCGATGCTGGGCTATACCAAATCGACGGTTTTGGTAT

Last Updated on 06.12.2018 16:11