Sequences from confirmation amplicon


Confirmation sequences for line 880H12 and hit At3g26520 are shown.
Line ID 880H12
Locus At3g26520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9723395
Primers used for PCR KQ80 - 8474, show primer details
   
Sequence Name 12-K103223-0022-880-H12C-8409
GenBank Accession LN491534 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K103223-0022-880-H12C-8409
TCGGACATGAGCCATTTACATTGAATATATCCTGGATTGCTCAGCTTCTTGGTATTCTCT
ACTGGATTGCTCAGCTTCTTGGCTCCGTCGCCGCTTGTTTCCTCCTTAGCTTTGCCACCG
GTGGCGAGGTAACGTCACTTACACCTAATTAGATTTTAGTAGTATTTTCGATTCTCTGTT
TCTCTATTTTGTGGTTTGGTTTAAAACTAAATTCAATTGAATATTCCAACTAAAATAACC
CGTTTGACCTAACTTCTCCGGCAAGTAAATAAACCTTTTTGCAAATGATTGTTTTTTCAG
CCAATTCCAGCGTTCGGTCTCTCTGCCGGAGTCGGATCATTAAACGCTCTCGTCTTCGAG
ATCGTGATGACCTTCGGGCTCGTCTACACCGTCTACGCCACAGCCGTTGACCCCAAGAAC
GGTAGTCTCGGAACAATCGCACCAATCGCCATAGGTTTCATCGTTGGAGCTAACATCCTC
GCCGGTGGAGCTTTCAGCGGAGCTTCCATGAACCCAGCCGTTGCTTTCGGACCAGCCGTC
GTAAGCTGGACGTGGACCAACCACTGGGTTTACTGGGCTGGTCCTCTTATTGGTGGTGGA
CTCGCCGGAATTATCTACGACTTTGTCTTCATCGATGAAAATGCCCACGAGCAATTGCCT
ACCACCGATTACTGAAGACTTCAAATTC
   
Sequence Name 60-K103230-0022-880-H12C-KQ80
GenBank Accession LN510231 [GenBank]
Sequencing primer gene specific primer, KQ80: 5'-TGAATTTGACGTCTTCAGTAATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K103230-0022-880-H12C-KQ80
CTTTTCTCGATGAAGACAAGTCGTAGATAATTCCGGCGAGTCCACCACCAATAAGAGGAC
CAGCCCAGTAAACCCAGTGGTTGGTCCACGTCCAGCTTACGACGGCTGGTCCGAAAGCAA
CGGCTGGGTTCATGGAAGCTCCGCTGAAAGCTCCACCGGCGAGGATGTTAGCTCCAACGA
TGAAACCTATGGCGATTGGTGCGATTGTTCCGAGACTACCGTTCTTGGGGTCAACGGCTG
TGGCGTAGACGGTGTAGACGAGCCCGAAGGTCATCACGATCTCGAAGACGAGAGCGTTTA
ATGATCCGACTCCGGCAGAGAGACCGAACGCTGGAATTGGCTGAAAAAACAATCATTTGC
AAAAAGGTTTATTTACTTGCCGGAGAAGTTAGGTCAAACGGGTTATTTTAGTTGGAATAT
TCAATTGAATTTAGTTTTAAACCAAACCACAAAATAGAGAAACAGAGAATCGAAAATACT
ACTAAAATCTAATTAGGTGTAAGTGACGTTACCTCGCCACCGGTGGCAAAGCTAAGGAGG
AAACAAGCGGCGACGGAGCCAAGAAGCTGAGCAATCCAGTAGAGAATACCAAGAAGCTGA
GCAATCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11