Sequences from confirmation amplicon


Confirmation sequences for line 881A04 and hit At3g51080 are shown.
Line ID 881A04
Locus At3g51080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18974204
Primers used for PCR 41uj - 8474, show primer details
   
Sequence Name 33-K117387-0022-881-A04C-41uj
GenBank Accession LN501161 [GenBank]
Sequencing primer gene specific primer, 41uj: 5'-AGAAAACCTAATTTGTGTTGGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K117387-0022-881-A04C-41uj
ACAGTAACATGAAAGACAAAACCCTAACCGGCGGCGCTCAAAATGGCGATGATTTCTCCG
TCGACGACTTGCTTGACTTCTCAAAAGAAGAAGAAGACGACGACGTTTTGGTTGAGGATG
AGGCTGAATTGAAAGTACAACGAAAAAGAGGAGTCTCTGACGAAAATACCCTTCACCGGA
GCAACGATTTCTCCACCGCTGATTTCCACACTAGCGGGCTTTCCGTTCCGGTATGAATTT
GTGTTTTCATTTTCGCAAAATTTATAAAAATTATCAGTCATTGCATTGTTAATTTCTTGC
TTTTCATGTTTTGATTGTTAGATGGATGATATAGCGGAACTTGAATGGTTATCAAATTTC
GTAGATGATTCTTCTTTCACGCCGTATTCTGCTCCGACGAATAAACCGGTTTGGTTAACC
GGAAATCGGAGACATCTTGTACAACCGGTTAAAGAGGAGACCTGCTTCAAATCCCAACAT
CCGGCCGTCAAAACCAGACCCAAACGAGCCAGAACCGGAGTCAGAGTCTGGTCTCATGTC
AGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCG
   
Sequence Name 06-K117386-0022-881-A04C-8409
GenBank Accession LN488988 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K117386-0022-881-A04C-8409
ATCCTGACATGAGACCAGACTCTGACTCCGGTTCTGGCTCGTTTGGGTCTGGTTTTGACG
GCCGGATGTTGGGATTTGAAGCAGGTCTCCTCTTTAACCGGTTGTACAAGATGTCTCCGA
TTTCCGGTTAACCAAACCGGTTTATTCGTCGGAGCAGAATACGGCGTGAAAGAAGAATCA
TCTACGAAATTTGATAACCATTCAAGTTCCGCTATATCATCCATCTAACAATCAAAACAT
GAAAAGCAAGAAATTAACAATGCAATGACTGATAATTTTTATAAATTTTGCGAAAATGAA
AACACAAATTCATACCGGAACGGAAAGCCCGCTAGTGTGGAAATCAGCGGTGGAGAAATC
GTTGCTCCGGTGAAGGGTATTTTCGTCAGAGACTCCTCTTTTTCGTTGTACTTTCAATTC
AGCCTCATCCTCAACCAAAACGTCGTCGTCTTCTTCTTCTTTTGAGAAGTCAAGCAAGTC
GTCGACGGAGAAATCATCGCCATTTTGAGCGCCGCCGGTTAGGGTTTTGTCTTTCATGTT
ACTGTTCTTCAACGTCAATTCAACACTCTCCATTATTTCCTATCAAACCCCACCAACACA
AATAAGGGTTTT

Last Updated on 06.12.2018 16:11