Sequences from confirmation amplicon


Confirmation sequences for line 881C01 and hit At4g34265 are shown.
Line ID 881C01
Locus At4g34265 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16402301
Primers used for PCR 16ed - 8474, show primer details
   
Sequence Name 19-K110172-0022-881-C01C-16ed
GenBank Accession LN494803 [GenBank]
Sequencing primer gene specific primer, 16ed: 5'-CAAAACTTTAAAAACTTGGCCACT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K110172-0022-881-C01C-16ed
GGAGAGTATTAGCGATCTTGACAAGTGCGTGGGAAGGTACCGTTAGAGAGTTCGCAGAAA
GCTCGCAGATTAACATTAAGGTGAGATGTGAGTCTCGCTGCAGCCACAACATTGTACAGA
GGCAAGAATGACTCTGTGCATCCTAGCTCTCCCAAATTCCTGTACACGATTACAAAACAT
GTTTCTTTCTGGCCAATGCTATCATAATCTCAATATGATAAAATCAAACATTTTTCAATT
CTTATCAACGGGAATTAGGGATTTCAATTTTAGAAATGGTGAAACCTGGGATTAGTGAAA
GAGGCAAATCGGCGGCGGGGCAAAGAGGATTGGGAAGGACGGAGGCGGGGAAGCGTTGGC
GGCGGAGATCGGAGAGACGGAGCTCTAGCTGTAGCGGAGACGAAAGAGCGTGCTGCACTG
CGCCATGCCATTTTTTATCTACTTGTAGCAGAAAGAAAACAACAAAGCAAATCAGTAAAT
AAACAAAAATGCAAATGTAGAAATCTACATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 03-K110171-0022-881-C01C-8409
GenBank Accession LN487533 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K110171-0022-881-C01C-8409
TTACATTGAAGTAGATTTCTACATTTGCATTTTTGTTTATTTACTGATTTGCTTTGTTGT
TTTCTTTCTGCTACAAGTAGATAAAAAATGGCATGGCGCAGTGCAGCACGCTCTTTCGTC
TCCGCTACAGCTAGAGCTCCGTCTCTCCGATCTCCGCCGCCAACGCTTCCCCGCCTCCGT
CCTTCCCAATCCTCTTTGCCCCGCCGCCGATTTGCCTCTTTCACTAATCCCAGGTTTCAC
CATTTCTAAAATTGAAATCCCTAATTCCCGTTGATAAGAATTGAAAAATGTTTGATTTTA
TCATATTGAGATTATGATAGCATTGGCCAGAAAGAAACATGTTTTGTAATCGTGTACAGG
AATTTGGGAGAGCTAGGATGCACAGAGTCATTCTTGCCTCTGTACAATGTTGTGGCTGCA
GCGAGACTCACATCTCACCTTAATGTTAATCTGCGAGCTTTCTGCGAACTCTCTAACGGT
ACCTTCCCACGCACTTGTCAAGATCGCTAATTACTCTCCATTTGTGAGTGTTACTGCTCT
TAGGATTTCATTTGGAGTGGCCAAGTTTTTAAAGTTTTG

Last Updated on 06.12.2018 16:11