Sequences from confirmation amplicon


Confirmation sequences for line 881C07 and hit At3g59845 are shown.
Line ID 881C07
Locus At3g59845 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22106651
Primers used for PCR 34qp - 8474, show primer details
   
Sequence Name 34-K116321-0022-881-C07C-34qp
GenBank Accession LN501556 [GenBank]
Sequencing primer gene specific primer, 34qp: 5'-CAAAAGGTTAGGCCATAGTTTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K116321-0022-881-C07C-34qp
TTACATAATTTGGTTTCCACAAAAGGTCGATCCTCCCTGCTGAACAAATTCGGTTACGAT
GATGCATTCAATTATAAGGAAGAGCCTGACCTTGACTCCGCCTTGAAGAGGTTCATATTT
GGCTTCTTATAAATCAGTTTCTTTGAATCATTGAATCAAAATCTCCTCACAATATTTCTC
TCTGCAGGTGTGTACCAAAAGGTATTGACATATACTTTGAGAACGTGGGAGGCAAAATGC
TTGACGCGGTTCTACTTAACATGAAAACGTATGGACGAATCGCTGTATGTGGGATGATAT
CTCAGTACCATCTTGAAACCAGAGACAGGTTACAAAATCTACCTGACATCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 02-K116320-0022-881-C07C-8409
GenBank Accession LN487188 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K116320-0022-881-C07C-8409
TGTACCTGTCTCTGGTTTCAAGATGGTACTGAGATATCATCCCACATACAGCGATTCGTC
CATACGTTTTCATGTTAAGTAGAACCGCGTCAAGCATTTTGCCTCCCACGTTCTCAAAGT
ATATGTCAATACCTTTTGGTACACACCTGCAGAGAGAAATATTGTGAGGAGATTTTGATT
CAATGATTCAAAGAAACTGATTTATAAGAAGCCAAATATGAACCTCTTCAAGGCGGAGTC
AAGGTCAGGCTCTTCCTTATAATTGAATGCATCATCGTAACCGAATTTGTTCAGCAGGAG
ATCGACCTTTTGTGGGAACCAAATTATGTAATTAGAGAAAACTTATATAGAGATAGTTTA
ACTCATTTGATTGATGAGTTTCAAAACTATGGCTAAAACCTTTTG

Last Updated on 06.12.2018 16:11