Sequences from confirmation amplicon


Confirmation sequences for line 888H04 and hit At4g37530 are shown.
Line ID 888H04
Locus At4g37530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17635998
Primers used for PCR 14hz - 8474, show primer details
   
Sequence Name 78-K112969-0022-888-H04C-14hz
GenBank Accession LN513712 [GenBank]
Sequencing primer gene specific primer, 14hz: 5'-GCCATGTTGTTAGCGGTTTATATT-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K112969-0022-888-H04C-14hz
GGCACACACATTAGGATTCGCGCATTGCACAAAAGTTTTCAATCGGCTATACAATTTCAA
CAAGACAAATAACGTGGATCCCACGATTAACAAAGATTACGTGACAGAGCTAAAAGCGTC
GTGTCCTCAAAACATAGATCCAAGAGTGGCTATTAACATGGATCCCAATACACCAAGACA
ATTCGACAACGTTTACTACAAAAACTTGCAACAAGGGAAAGGATTGTTCACGTCTGATCA
AGTCTTATTCACCGATAGTCGGTCAAAGCCAACCGTTGACTTATGGGCTAATAATGGACA
GTTGTTTAATCAAGCTTTTATTCAATTGAATATTTACAGTTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 71-K112968-0022-888-H04C-8409
GenBank Accession LN512585 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K112968-0022-888-H04C-8409
ATTTCACTGTAATATTCAATTGAATAAAAGCTTGATTAAACAACTGTCCATTATTAGCCC
ATAAGTCAACGGTTGGCTTTGACCGACTATCGGTGAATAAGACTTGATCAGACGTGAACA
ATCCTTTCCCTTGTTGCAAGTTTTTGTAGTAAACGTTGTCGAATTGTCTTGGTGTATTGG
GATCCATGTTAATAGCCACTCTTGGATCTATGTTTTGAGGACACGACGCTTTTAGCTCTG
TCACGTAATCTTTGTTAATCGTGGGATCCACGTTATTTGTCTTGTTGAAATTGTATAGCC
GATTGAAAACTTTTGTGCAATGCGCGAATCCTAATGTGTGTGCCCCTGAGATGTTTAAGA
GCAAACGAAATCTAAATTTGGACCGGAAATATAAACCGCTAACAACATGG

Last Updated on 06.12.2018 16:11