Sequences from confirmation amplicon


Confirmation sequences for line 889D10 and hit At1g77100 are shown.
Line ID 889D10
Locus At1g77100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28966112
Primers used for PCR 17my - 8474, show primer details
   
Sequence Name 27-K114505-0022-889-D10C-17my
GenBank Accession LN498500 [GenBank]
Sequencing primer gene specific primer, 17my: 5'-ATTCCAATATGGTGGGATTGAATA-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K114505-0022-889-D10C-17my
ATAATTGGTATAAATATATCTTTTCATAATTCAAATTTGTTTATTTTGTTAAACCAACAA
ACTCAAATTGTTATACTTAACTATGTATGAGATCTTGGAAACTAGTTTGTAGCACTGCAT
AAACGTCTAATCTCTCCTTCAGCACCGATTTTCACTCCGATAGCACCCATCTTTATCATG
GCTTTAGTGAAATCCGCAGCAAAATTAGCCTTTGAACTTTGATTAGTTTCTAAGTATGAA
TCAATGATTTTCTTCATATTATTGTCTTGGTATAAAACAGAGTCCGATAGAATGACACCT
CTGCCATTTTTGATGTTTTGGAAGATTTGGTTATCAAACACAAATTGGCTATCCCAGTCC
AACGGAATCCTGACATTAACATCACCGCCTTGGGGACACTTTGACCTCAGGATTTGAAAA
AATTCCGGGTTTATCGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 02-K114504-0022-889-D10C-8409
GenBank Accession LN487134 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K114504-0022-889-D10C-8409
ATTTCATTGAATATATCCTGCGATAAACCCGGAATTTTTTCAAATCCTGAGGTCAAAGTG
TCCCCAAGGCGGTGATGTTAATGTCAGGATTCCGTTGGACTGGGATAGCCAATTTGTGTT
TGATAACCAAATCTTCCAAAACATCAAAAATGGCAGAGGTGTCATTCTATCGGACTCTGT
TTTATACCAAGACAATAATATGAAGAAAATCATTGATTCATACTTAGAAACTAATCAAAG
TTCAAAGGCTAATTTTGCTGCGGATTTCACTAAAGCCATGATAAAGATGGGTGCTATCGG
AGTGAAAATCGGTGCTGAAGGAGAGATTAGACGTTTATGCAGTGCTACAAACTAGTTTCC
AAGATCTCATACATAGTTAAGTATAACAATTTGAGTTTGTTGGTTTAACAAAATAAACAA
ATTTGAATTATGAAAAGATATATTTATACCAATTATTGTAGCATTGCATATACGTCTATN
AATCTATTAGATATTCAATCCCACCTAATTGGA

Last Updated on 06.12.2018 16:11