Sequences from confirmation amplicon


Confirmation sequences for line 889F04 and hit At1g13430 are shown.
Line ID 889F04
Locus At1g13430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4607620
Primers used for PCR 31my - 8474, show primer details
   
Sequence Name 35-K114505-0022-889-F04C-31my
GenBank Accession LN501930 [GenBank]
Sequencing primer gene specific primer, 31my: 5'-CAAAAGTTACTGAGACGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K114505-0022-889-F04C-31my
GATCAGCCAGTTTGTTGAACGGAGGATCAAAAATTGGAATTTAGTATTTCCTTGCAATTG
CGAAGATCCAATTTTTTGGTTGACATCATTGTAAAATGATTGATATTATAATTTTATTTG
CATGCTAACTTTTAAAGTTAATAGGAAACTCGTATTTTCAATAGTGACATGTTATTGCAT
GTGATAATACGATTATTTTATTACTTTATTAGTCGTAAATGAAGAGTAACATGCCACTAT
TGAAATGGTACTCATGAGATCACACGCTAGTTATTAAAGGAACATAGAAGTAAAGAAGGT
TTTTATTGCAACATCAAAGAGAACAAAAACCACACATCAGTTGTAATGATACATCAAAGT
TATATATATGACAATGTAACTTTTATTATTAAATAACGCATTCAAGAACACTTACAATTG
ATAAAACTACAATAGAATATGAAAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTC
   
Sequence Name 10-K114504-0022-889-F04C-8409
GenBank Accession LN490762 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K114504-0022-889-F04C-8409
ATTTTTCAATTCTATTGTAGTTTTATCAATTGTAAGTGTTCTTGAATGCGTTATTTAATA
ATAAAAGTTACATTGTCATATATATAACTTTGATGTATCATTACAACTGATGTGTGGTTT
TTGTTCTCTTTGATGTTGCAATAAAAACCTTCTTTACTTCTATGTTCCTTTAATAACTAG
CGTGTGATCTCATGAGTACCATTTCAATAGTGGCATGTTACTCTTCATTTACGACTAATA
AAGTAATAAAATAATCGTATTATCACATGCAATAACATGTCACTATTGAAAATACGAGTT
TCCTATTAACTTTAAAAGTTAGCATGCAAATAAAATTATAATATCAATCATTTTACAATG
ATGTCAACCAAAAAATTGGATCTTCGCAATTGCAAGGAAATACTAAATTCCAATTTTTGA
TCCTCCGTTTCAACAAACTTGGCTGATTCGTTAAAAAACAATGTGGCGATGCTTATCAAC
CAATAAATCCGTCTCATAAACTTTT

Last Updated on 06.12.2018 16:11