Sequences from confirmation amplicon


Confirmation sequences for line 889G02 and hit At3g56330 are shown.
Line ID 889G02
Locus At3g56330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20891526
Primers used for PCR 43vf - 8474, show primer details
   
Sequence Name 48-K117776-0022-889-G02C-43vf
GenBank Accession LN506629 [GenBank]
Sequencing primer gene specific primer, 43vf: 5'-AATCTGTTTTAAGAGCATTGGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K117776-0022-889-G02C-43vf
CAAAATCCAGGAGCAGAGCTTTTTACTTTGTACCTTCAGATCAGTTTCTTCAGAGATCAA
AGCTGTGAGAATCTAACTACTATTTACCTTAACTAGTGCACTCATCATCTTCTTCAACGG
AGGTGAGTTCATCTTCGCACGGCTCGCCATCTAATCACGAAAACAAATCTTTTATCATGT
AATGTGAATGAAATGCGTCTATCTTATCAATGAAATTAAAATCATGGTTTTAGAGGACCT
CATCCATTTTTGTGTATCCAGGTGGTAGTCTGGGATCACTTTCTTCAATCATTATACAAA
AATGTAGATGTCCATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCACAGTTATTAT
   
Sequence Name 24-K117775-0022-889-G02C-8409
GenBank Accession LN497169 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K117775-0022-889-G02C-8409
TCATGGACATCTACATTTTTGTATAATGATTGAAGAAAGTGATCCCAGACTACCACCTGG
ATACACAAAAATGGATGAGGTCCTCTAAAACCATGATTTTAATTTCATTGATAAGATAGA
CGCATTTCATTCACATTACATGATAAAAGATTTGTTTTCGTGATTAGATGGCGAGCCGTG
CGAAGATGAACTCACCTCCGTTGAAGAAGATGATGAGTGCACTAGTTAAGGTAAATAGTA
GTTAGATTCTCACAGCTTTGATCTCTGAAGAAACTGATCTGAAGGTACAAAGTTAAAAAG
CTCTGCTCCTTGGATTTTTGCAGGAGGGTTACGCTGCTAGCAGATCTCACATTATTCCCA
ATGCTCTTAAAACAGATT

Last Updated on 06.12.2018 16:11