Sequences from confirmation amplicon


Confirmation sequences for line 890C10 and hit At1g31820 are shown.
Line ID 890C10
Locus At1g31820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11417303
Primers used for PCR NB11 - 8474, show primer details
   
Sequence Name 72-K101328-0022-890-C10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K101328-0022-890-C10C-8409
CCATTTACAACGGAGGTCAGCAATAAATACTCATTGTAACCCATAACTCACGATCCAACG
NGTATAGCCCCGGGTTCCAGACAAAAGAAGGGAGGAAAGTTCGAAAGAAGCGACAAANGA
TAACACCNGTAACACANAAGCCTTAGGGAAGTGGTTTTCTTCGGAAATTTGCCACTTCAN
NCCGGCCAGTGTACNNTAACCGAATCCCAGTAGTTCAGAATTCCATAAANAGCGTGGNTT
GAGATACANAGTTCCANGTTGAACATTTCCTAAATCCNATCACAANNGCCATCTCGAAGG
GCTNNCGAGCTGCGGAATANGAAACCAGAAACTCATTANNCAGCAAACGGANNAGCATAG
AGAANNAACTCCCATAAACANNCAGCGGTCCANNACCAACAATGGGGTTAGTCCNNTCTA
TAGTNNNTAAGTAAGTANNAGCAATAGAAAGTCAAGAATCAAGAATNNCGATGCANNACT
CTTGGGCAAACCCNNAGTAGCTNNAAAGCCGGGGAACCGCGNGAACTTCNNAAATAATCG
AGGAAAAAAGAACAGGGAATACAAAGCGTTATCAATNNAACACCGCANNAGCCATNNTTC
ATCCAACCTACTNNTGAAACCCCCAAAAGGGGTTCCCAAAAGCA
   
Sequence Name 72-K101334-0022-890-C10C-NB11
GenBank Accession LN512691 [GenBank]
Sequencing primer gene specific primer, NB11: 5'-AGTACAATGTTTCCAATAAACGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K101334-0022-890-C10C-NB11
CTTTGGGACCTTTTGGGGGTTTCAAGTAGGTTGGATGAAATGGCTTTGCGGTGTTATTGA
TAACGCTTTGTATCCTGTTCTTTTCCTCGATTATTTGAAGTCCGCGGTCCCGGCTTTAGC
TACTGGTTTGCCAAGAGTTGCATCGATCTTGATCTTGACTCTATTGCTTACTTACTTAAA
CTATAGAGGACTAACCATTGTTGGTTGGACCGCTGTGTTTATGGGAGTTTTCTCTATGCT
TCCGTTTGCTGTAATGAGTCTGGTTTCTATTCCGCAGCTCGAGCCTTCGAGATGGCTTGT
GATGGATTTAGGAAATGTCAACTGGAACTTGTATCTCAACACGCTTTTATGGAATCTGAA
CTACTGGGATTCGGTTAGTACACTGGCCGGTGAAGTGGCAAATCCGAAGAAAACACTTCC
TAAGGCTTTGTGTTACGGTGTTATCTTTGTCGCTCTTTCGAACTTCCTCCCTCTTTTGTC
TGGAACCGGGGCTATACCGTTGGATCGTGAGTTATGGGTTACAATGAGTATTTATTGCTG
ACCTCCGTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCA

Last Updated on 06.12.2018 16:11