Sequences from confirmation amplicon


Confirmation sequences for line 891E05 and hit At5g10520 are shown.
Line ID 891E05
Locus At5g10520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3322574
Primers used for PCR 29pi - 8474, show primer details
   
Sequence Name 42-K115005-0022-891-E05C-29pi
GenBank Accession LN504569 [GenBank]
Sequencing primer gene specific primer, 29pi: 5'-TGAGTTCTTTACATCGTAAATGCG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K115005-0022-891-E05C-29pi
CTTCCTCAAATTANAAAATCATAGGACTACTAATTTAGAAACAAGAAATTACAAAAAACA
AAACAAGAAAAACAAAACTTGACTTGACTTGCAGTTGAAAGTAAAAAATAAAAACAAAAG
TTGAATTGCTACGGTGGAGTTGTATAAATATAGCAGAGATTCCAATCCATCTCCACTCAG
CAAAAGCTGACTCTTCTTCTAACTTTCTATTTCTTCCAAGAATAACAAACTTTTTTCTCA
TAAGAAACAAAACAAGAATTCTACAAAACTTCTCATTAAACCTCTGTTTTCTTGTTTCTC
TTCTGCTCTGTATTTTCGATAATTCTCTCTGTTTTGTATCCTTTCTTATTTTCTGTGAAT
CTGTTTTTTTATGGAAATTTAATTCAAAGGCTTATTAGAGGAAGAAAGAAAGAAAAAGAA
AAAAAAACCCACTGGAGCTATGGCTGTTGAAGGTAAACCTAAACATAAAATTTGTTTGCT
TTTTGTTAAGAAAGTGGGGGAAAAAAATAAAATCAGATATATTCAATGGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATT
   
Sequence Name 10-K115004-0022-891-E05C-8409
GenBank Accession LN490780 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K115004-0022-891-E05C-8409
CATTTACATTGAATATATCTGATTTTATTTTTTTCCCCCACTTTCTTAACACAATAGCAA
ACAAATTTTATGTTTAGGTTTACCTTCAACAGCCATAGCTCCAGTGGGTTTTTTTTTCTT
TTTCTTTCTTTCTTCCTCTAATAAGCCTTTGAATTAAATTTCCATAAAAAAACAGATTCA
CAGAAAATAAGAAAGGATACAAAACAGAGAGAATTATCGAAAATACAGAGCAGAAGAGAA
ACAAGAAAACAGAGGTTTAATGAGAAGTTTTGTAGAATTCTTGTTTTGTTTCTTATGAGA
AAAAAGTTTGTTATTCTTGGAAGAAATAGAAAGTTAGAAGAAGAGTCAGCTTTTGCTGAG
TGGAGATGGATTGGAATCTCTGCTATATTTATACAACTCCACCGTAGCAATTCAACTTTT
GTTTTTATTTTTTACTTTCAACTGCAAGTCAAGTCAAGTTTTGTTTTTCTTGTTTTGTTT
TTTGTAATTTCTTGTTTCTAAATTAGTAGTCCTATGATTTTAATAATTTTGAGGAAGATG
AAAGGATTAGAGATTGTTGCCGTAACTAACGCATTTACAATTAAAGAAACTC

Last Updated on 06.12.2018 16:11