Sequences from confirmation amplicon


Confirmation sequences for line 891G03 and hit At5g02620 are shown.
Line ID 891G03
Locus At5g02620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:591381
Primers used for PCR 44pf - 8474, show primer details
   
Sequence Name 67-K115259-0022-891-G03C-44pf
GenBank Accession LN511877 [GenBank]
Sequencing primer gene specific primer, 44pf: 5'-ATCAAGCCAGCTGAAAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K115259-0022-891-G03C-44pf
TTAGTGAATAGGCCATGAGGTGCATACTCAGCTTGAACAAACCGGGAGAACGAGAAGAGA
GATCCAAGGAATCGCTAAACGGGTCAACAAAATGCACACGGAAGGACTCAACAACGCGAT
AAACTCCACTACCCTAGTAGCCATTCTCATAGCAACCGTTGCTTTTGCAGCTATCTTCAA
CGTTCCAGGCCAGTACACGGACGACCCTAAAGACGTACCTCCAGGATATTCACTCGGAGA
AGCAAGAGCAGCTCCAAGACCCGAGTTCTTGATATTCGTCGTGTTTGATTCTTTCGCGCT
TTTCATCTCTTTAGCGGTGGTTGTGGTTCAGACATCAGTGGTGGTTATAGAGAGGAGAGC
AAAGAAGCAAATGATGGCTATNATCAACAAGCTCATGTGGATGGCTTGCATTATGATCTC
AGTAGCGTTCGTATCACTGTCTTTTGTTGTCGTGGGAGAGAAAGAGAAGCCTTTGGCGGT
TGGAGTAACCGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCG
   
Sequence Name 27-K115258-0022-891-G03C-8409
GenBank Accession LN498522 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K115258-0022-891-G03C-8409
TTGAATATATCCGGTTACTCAACCGCCAAAGGCTTCTCTTTCTCTCCCACGACAACAAAA
GACAGTGATACGAACGCTACTGAGATCATAATGCAAGCCATCCACATGAGCTTGTTGATT
ATAGCCATCATTTGCTTCTTTGCTCTCCTCTCTATAACCACCACTGATGTCTGAACCACA
ACCACCGCTAAAGAGATGAAAAGCGCGAAAGAATCAAACACGACGAATATCAAGAACTCG
GGTCTTGGAGCTGCTCTTGCTTCTCCGAGTGAATATCCTGGAGGTACGTCTTTAGGGTCG
TCCGTGTACTGGCCTGGAACGTTGAAGATAGCTGCAAAAGCAACGGTTGCTATGAGAATG
GCTACTAGGGTAGTGGAGTTTATCGCGTTGTTGAGTCCTTCCGTGTGCATTTTGTTGACC
CGTTTAGCGATTCCTTGGATCTCTCTTCTCGTTCTCCCGGTTTGTTCAAGCTGAGTATGC
ACCTCATGGCCTATTTCACTAACTGTTTCTTTCAGTTTCCTAGAAGAGCCTGATGGTTCC
ACTTTTTCAGCGNGCTTGA

Last Updated on 06.12.2018 16:11