Sequences from confirmation amplicon


Confirmation sequences for line 893C02 and hit At4g34570 are shown.
Line ID 893C02
Locus At4g34570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16512130
Primers used for PCR 23kw - 8474, show primer details
   
Sequence Name 52-K113872-0022-893-C02C-23kw
GenBank Accession LN507882 [GenBank]
Sequencing primer gene specific primer, 23kw: 5'-TCATCAGGATTGTTCTTGATCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K113872-0022-893-C02C-23kw
AGTATAATCAGCATGCATATCTGTGTACCTGTAGAGAGAGATGAACAAAATGTCTCGACA
CAGAAATCTGTTTTGCCAAAACTCAGAAAGTGTCAAGAGATAAGAGAGAAGCTACTGACT
TAGCACCAAAGTGTCTCCACTGAAATCCATAAACGGGTCCAAGGTCACCTTCCTCTCTCT
CAGTCAGTCCAATACTGAAATTAAAAACTTTCAATTTATCAGTGTAATGGCCACAAAGCT
TCAAGTTGTGAAAGAAAAATGCTGAATTAAAGTTTACCCATCAAGATATGCTCTCGACGC
ATTCCCATCCCATATACCCATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 26-K113871-0022-893-C02C-8409
GenBank Accession LN498017 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K113871-0022-893-C02C-8409
TCATTGAATATATCCTGATGGGTATATGGGATGGGAATGCGTCGAGAGCATATCTTGATG
GGTAAACTTTAATTCAGCATTTTTCTTTCACAACTTGAAGCTTTGTGGCCATTACACTGA
TAAATTGAAAGTTTTTAATTTCAGTATTGGACTGACTGAGAGAGAGGAAGGTGACCTTGG
ACCCGTTTATGGATTTCAGTGGAGACACTTTGGTGCTAAGTCAGTAGCTTCTCTCTTATC
TCTTGACACTTTCTGAGTTTTGGCAAAACAGATTTCTGTGTCGAGACATTTTGTTCATCT
CTCTCTACAGGTACACAGATATGCATGCTGATTATACTGGCCAAGGATTTGATCAGCTCT
TAGATGTAATTAACAAGATCAAGAACAATCCTGATG

Last Updated on 06.12.2018 16:11