Sequences from confirmation amplicon


Confirmation sequences for line 894H01 and hit At4g15920 are shown.
Line ID 894H01
Locus At4g15920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9031221
Primers used for PCR 31kk - 8474, show primer details
   
Sequence Name 71-K113845-0022-894-H01C-31kk
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 31kk: 5'-AGTAGTGACAACAAAGAGCGTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K113845-0022-894-H01C-31kk
CTGAATAATTACTGAGGAGAGGCTGTTGAGTGGTTAAATTTCCGATATTGCAACCCCAAT
   
Sequence Name 44-K113844-0022-894-H01C-8409
GenBank Accession LN505254 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K113844-0022-894-H01C-8409
CATTTCATTGAATATATCCTGACACTAATGGGGTCTAATTGGGGATGTAAAGAAAACAAC
AAAAATATTTTGGGGGTATTGCTGGAATATCGAAAAAGATATGGGGTCGATGAAATAGCT
TTGTTAAGTTTTCCTTTTTGTCTTTTGAATGTGTCTAAAGCCAAAACACTAACGGGACAC
AGAGACGGCATGAGTATAAGGGTGTAATCATAAATTAAGAAGTGAATAAAACAGTGAAAC
TATACTTACGAGTAAGAAAACGTCGTGTTGGAGTAAAGCATAGACAGCCCAAATCGCGCC
GTTTAAGAAGAGGAAAAACGACAACCAAAACGGCATGTATTTCACGCTCTTTGTGNTCAC
TAC

Last Updated on 06.12.2018 16:11