Sequences from confirmation amplicon


Confirmation sequences for line 895B06 and hit At5g16730 are shown.
Line ID 895B06
Locus At5g16730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5497665
Primers used for PCR 12vj - 8474, show primer details
   
Sequence Name 42-K117776-0022-895-B06C-12vj
GenBank Accession LN504622 [GenBank]
Sequencing primer gene specific primer, 12vj: 5'-TTCAATCTCAGAATTCTCCTCCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K117776-0022-895-B06C-12vj
TTTAGCATCATCCGTTCGCCAATGAAGATGTCCGCTCCTTCGCTTTCTTCAAATCTTCCT
TGATCTGACTCAACCGTGTGGTCGTCTGCGGTGATTCTGTTCCTTTCACCGCTGCTACTC
GCGCTTGAGATTTCTGCAAATCATCAGCAAACAAAATTGATATTAAAGAAACATTTTCAG
TCGAAGCAACTCCAAGATAAACACAAATGGCAAAACTTTTAACTACCTCAGGAGGAGTTG
GAAGCTTTGGTGATCGTCTCTCAACAGAAGATTTGGAATTTGGAGAAGAACGATCAAGAG
AAAGACGTGAATGTGGAGTAGTAGTAGATGGAGAATTATTATTACTAGTTTCGGATTTAT
TAACTGTTCTTTTTGCTATTCTTGGAGTTGCTGGAGATGATTTCCCGGTAGGAGTAGTAG
TAGTTGTTGTTTCGGACAAACTAGTTCTGTTTCCAACAAAAGACAATAACAACAGTCGAT
CTCAGAACAAAGAAATAAATTGCCGCAAAAAATTAGGACAAAACAAATGTAGAAATGAAT
TTAGTAGATCCAAAACTCGATCATTGACAATTTCTCAAAATAGTAACTTGGATCAGATCT
ACTACGATCTACTAAAAAATTTCCCAGAGAAAATAGGATAAAACTAAAACAGAGTGAAAC
TGAGTAAAGAGTCTTACTTAGTCTTCGAAGCCATAACCGTCGATCGCTGTCACCGTCGCC
ACCACCGTCGCCACCACCGTCGCGCTCAGTTGATCAGAAATTCGATTGATAATTTTGCTG
GAATTGGGACTNCATTTTCAGATCAAGAAAGAGAGAGAGAATGATTCGAATCGGTGGGAT
CGAAGTAACGAAGAATGATATAGATNATGGCAGGTGTGGTTGTCT
   
Sequence Name 18-K117775-0022-895-B06C-oriL
GenBank Accession LN494492 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K117775-0022-895-B06C-oriL
TCGGATTTTCTGCTACCGATCATATTGGCTTCTTTTTACGAGTTAGTGAGGCCACTCTGG
AGACAACCACACCTGCCATTATCTATATCATTCTCCGTTACTTCGATCCCACCGATTCGA
ATCATTCTCTCTCTCTTTCTTGATCTGAAAATTGAGTCCCAATTCCAGCAAAATTATCAA
TCGAATTTCTGATCAACTGAGCGCGACGGTGGTGGCGACGGTGGTGGCGACGGTGACAGC
GATCGACGGTTATGGCTTCGAAGACTAAGTAAGACTCTTTACTCAGTTTCACTCTGTTTT
AGTTTTATCCTATTTTCTCTGGGAAATTTTTTAGTAGATCGTAGTAGATCTGATCCAAGT
TACTATTTTGAGAAATTGTCAATGATCGAGTTTTGGATCTACTAAATTCATTTCTACATT
TGTTTTGTCCTAATTTTTTGCGGCAATTTATTTCTTTGTTCTGAGATCGACTGTTGTTAT
TGTCTTTTGTTGGAAACAGAACTAGTTTGTCCGAAACAACAACTACTACTACTCCTACCG
GGAAATCATCTCCAGCAACTCCAAGAATAGCAAAAAGAACAGTTAATAAATCCGAAACTA
GTAATAATAATTCTCCATCTACTACTACTCCACATTCACGTCTTTCTCTTGATCGTTCTT
CTCCAAATTCCAAATCTTCTGTTGAGAGACGATCACCAAAGCTTCCAACTCCTCCTGAGG
TAGTTAAAAGTTTTGCCATTTGTGTTTATCTTGGAGTTGCTTCGACTGAAAATGTTTCTT
TAATATCAATTTTGTTTGCTGATGATTTGCAGAAATCTCAAGCGCGAGTAGCAGCGGTGA
AAGGAACAGAATCACCGCAGACGACCACACGGTTGAGTCAGATCAAGGAAGATT
Primers used for PCR 17vk - 8474, show primer details
   
Sequence Name 41-K117776-0022-895-B06C-17vk
GenBank Accession LN504241 [GenBank]
Sequencing primer gene specific primer, 17vk: 5'-AAACGTCATGATTATACTTTGGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K117776-0022-895-B06C-17vk
TCACATGCCCATTTTACTTTTGCATTCCTAATAAATAAGATGTAAATAAATAAATAAAAC
CTAGTATATATCATGACAATAATAGTTTCTTTGCGATAATGATTGATGGTAATGAATTGT
ATAGTTTTATTTTGAAGTATATAATGAAGAATGTATAGTTTTACCAAACAAAATCATTGA
ATCATACCAAATATTTTTGCCAAAATGATTTATATATTTTATTTCTTATGAACGCATTTA
TATACCAACCGTATAATAATAAATAGGAAACACGGAAACGTTGACAAAAAAGAAAATGAC
GAACAATAAGAACCGTTGATCTTGTTAGAAGCCGCAATGATATGAACAAGAAGAAGAGAG
TGACGGTAGTTTCGCTGTCAGTTTGATTGGACAAAAGAAGAGTGTGGGTCATTATCGTCA
TTTTACCAACCTTCACGTTCTCTTAATTTCTATTTTACCATTATTTAATGAAGCTAGATT
ATTAGTGGTTTCTTTGTTTTTATACATCGGATCGGTAGCAGGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGATTA
TTA
   
Sequence Name 17-K117775-0022-895-B06C-8409
GenBank Accession LN494042 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K117775-0022-895-B06C-8409
TTGAATATATCCTGCTACCGATCCGATGTATAAAAACAAAGAAACCACTAATAATCTAGC
TTCATTAAATAATGGTAAAATAGAAATTAAGAGAACGTGAAGGTTGGTAAAATGACGATA
ATGACCCACACTCTTCTTTTGTCCAATCAAACTGACAGCGAAACTACCGTCACTCTCTTC
TTCTTGTTCATATCATTGCGGCTTCTAACAAGATCAACGGTTCTTATTGTTCGTCATTTT
CTTTTTTGTCAACGTTTCCGTGTTTCCTATTTATTATTATACGGTTGGTATATAAATGCG
TTCATAAGAAATAAAATATATAAATCATTTTGGCAAAAATATTTGGTATGATTCAATGAT
TTTGTTTGGTAAAACTATACATTCTTCATTATATACTTCAAAATAAAACTATACAATTCA
TTACCATCAATCATTATCGCAAAGAAACTATTATTGTCATGATATATACTAGGTTTTATT
TATTTATTTACATCTTATTTATTAGGAATGCAAAAGTAAAATGGGCATGTGACAATTGGT
AAAGAACACATGAGATGAGACTAATGATTCCTTTATCTGCCCAAAGTATAACATGGACGT

Last Updated on 06.12.2018 16:11