Sequences from confirmation amplicon


Confirmation sequences for line 895E11 and hit At3g59970 are shown.
Line ID 895E11
Locus At3g59970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22153228
Primers used for PCR 67sm - 8474, show primer details
   
Sequence Name 60-K116491-0022-895-E11C-67sm
GenBank Accession LN510350 [GenBank]
Sequencing primer gene specific primer, 67sm: 5'-AGAACCTAACAGTGGTGCTCAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K116491-0022-895-E11C-67sm
ATTTCTCGTTCTCTCCTTGGAGACGCCCTGCAAATGTTTTCCGTACTAAGGAAGATGTTC
GCCCAATTTTCTGGTAACCTGCATTTTGTTTCTCTTGCTCGGAGTATTATTACAGATCTT
TAACTTTAAGATATATTTGCGGAGGAATAATACTGTTACTTCATTTTTTATCTTTGCAGG
GCAAACCGTCCAAAGAGCTACATATCTAGAACAAAGGGCTGGAATGACTTCCCACATGGA
CGTTGGGGTGATTCACACAGTGCAGCATACAGTACACTTTCGGATTATCAGGTACGTACC
ACATATTTATCAGTTTTCTGACATATCGACTTTATATTTTGAAAACTTGAATTCTGAAAG
GCTTGTTTTCAGTTTGCCTTCTCAATTTGCTTAAATTGTATTTGCATATTGTTATTTAGC
CCTACCATTATCACTTGTAAGAAGCTGCTTGCTTTGCTTGCCATCTCTTCTGCGTTTTTC
ATTGCCAATGAGTAGTGACAAGAAGCTTCAGCAACTTAAAACATTACAAATAGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
   
Sequence Name 28-K116490-0022-895-E11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K116490-0022-895-E11C-8409
GCCATTTACAATTGAATATATNCTATTTGTAATGGGTTTTAAGTTGNNCTGAAGCTTCNN
TTGTCACTACTCANTNNTGGCAATGAAAAANCGCANGAAGAAGATGNNGCAAGCAANNAG
CAAGCAGCTTCTTACAANGTGATAATGGGTANNGGGCTAAATAACANNATATGCAAATAC
NNAATTTAAGCANNAATTGAGAAGGCAAANCTGAAAACANNAGCCTTTCAGAAATTCAAN
GTTTTCAAANNATATAAAGTCGAAATATGTCAGAAAAAACTGATAANATATGNNTGGTAC
GTACCTGAAATAATCCGAAAAGTGTANNCTGTATGCTGNNCACTGTGTGAATCACNNCCC
AACGTCCNNATGTGGGAAGNNTCATTCCAGCCNNCTTTGTTCTAGAAATATGTAGNNCTC
TTTGGACNGGTTNNTGCCCTGCAAAGAAATAAAAANNATGAAGTAACNAGNTATTATTNN
NCCTCCGCAANNATATATCTTAANNAGTTAAAGAAATCTGTAATANNATACTCCGAAAAG
CAAGAAGAANNACAAAATNNGCAGGT

Last Updated on 06.12.2018 16:11