Sequences from confirmation amplicon


Confirmation sequences for line 895F02 and hit At3g06650 are shown.
Line ID 895F02
Locus At3g06650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2079479
Primers used for PCR KR54 - 8474, show primer details
   
Sequence Name 11-K100810-0022-895-F02C-8409
GenBank Accession LN491028 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K100810-0022-895-F02C-8409
GCCTTTACAATTGAAGGTCTAAGAAGAGAGATCCATGGCTCCATCGACGTTGAGTACCAG
GTTGTTGGCTTTAGACAGTGTGTATGTCTCTACTTGGACTGCGTATTCCATGTACTTCAC
TGCAGGGAAGTTAGACCGTGCAAATTTCTGAAGCAGCTCCACTCTTTTGTCTCTGTTGTC
TCTGCTCTTTATCCTACAAATATGTAAGAAAACAAATCCAGTCAGAGAATGTTCTGTTCC
TGCTATTGATAAAAAGGATACTTGATTGATATATAACGCCAATTTCCACATTTACATGTG
AAAACAAACGATGAAGATCTAAGCTCGTTTGTTTGTTCTTACCTGTGACCAATGCCAGGG
ACACGGATTCCTTTCTTCTTCATGCCTTCCACGAATTCATAAGGTGTGAGATTCTGAATA
GGGCACAACATTTTAGTACTATTTATATCTNAGTTTCAAGAAAGGCTTGATGCAGTTGCA
GTGAAGAATTAAAATG
   
Sequence Name 11-K100824-0022-895-F02C-KR54
GenBank Accession LN491030 [GenBank]
Sequencing primer gene specific primer, KR54: 5'-TCATTTTATCCTTCACTGCAACTG-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K100824-0022-895-F02C-KR54
TAATAGTACAAAATGTTGTGCCCTATTCAGAATCTCACACCTTATGAATTCGTGGAAGGC
ATGAAGAAGAAAGGAATCCGTGTCCCTGGCATTGGTCACAGGTAAGAACAAACAAACGAG
CTTAGATCTTCATCGTTTGTTTTCACATGTAAATGTGGAAATTGGCGTTATATATCAATC
AAGTATCCTTTTTATCAATAGCAGGAACAGAACATTCTCTGACTGGATTTGTTTTCTTAC
ATATTTGTAGGATAAAGAGCAGAGACAACAGAGACAAAAGAGTGGAGCTGCTTCAGAAAT
TTGCACGGTCTAACTTCCCTGCAGTGAAGTACATGGAATACGCAGTCCAAGTAGAGACAT
ACACACTGTCTAAAGCCAACAACCTGGTACTCAACGTCGATGGAGCCATTGGATCTCTCT
TCTTAGACCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGC
Primers used for PCR KR55 - 8474, show primer details
   
Sequence Name 19-K100811-0022-895-F02C-8409
GenBank Accession LN494675 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K100811-0022-895-F02C-8409
GCCATTTACAGAGATAGACGAAATAGTTCAGATCGGTTATCTCAACGGCCTCTTCGTCCT
CGCTCGATCCATCGGTTTAATCGGGTAACTGTCATAACCAAACACTTACACTTGTTCTTA
AAACAAATTTAAATTGATGTGGATTACGTGAATAAAATTGTTGCAGGCACACATTCGATC
AGAAGAGACTGAAGCAGCCACTGTACCGACACCCGTGGGAAGATGTCTTGTACACCAAGT
AATCATATCATTATTCACTTCTTCTGGTCGTTCCTTATTCCTGTTATCATTCTCTCCCAT
TCAGTTAGGCTATGTTTGATTATATATTTGTTCTGACACTCTATCTGTTGTGATCTCTTA
ATGCTTGAAAAAATAAAAGAAAGTTCAATTTTAGAAGAATTATAATGTTTTGATTAAATT
CTTTTAAAAAGTTCAATATAATCCTGACGTTTTATCAAATTTAAACTTTATGAATAGTTC
GAGTTAAACAAATAAGTCACCATATCCCACGACCAACAACTTATCTNAAAATATTTATGT
TGTTGTTTACCTCTGGAACATTATGAAGCTCTNATCTTCGACGCTNAGTTTTTGAATATN
TTCTAAAGAACCAATAACACTAGAATCAGTAAAATCCAAAAACAAAACAAAAATTATGAC
ACTTAAGCATGTTAGCATTTATGAGTGAGGAACATATCAAAGTATGGT
   
Sequence Name 19-K100825-0022-895-F02C-KR55
GenBank Accession LN494677 [GenBank]
Sequencing primer gene specific primer, KR55: 5'-ATACCGGCAACTAACCATACTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K100825-0022-895-F02C-KR55
TGCTACTGCTTAGTGTCATAATTTTTGTTTTGTTTTTGGATTTTACTGATTCTAGTGTTA
TTGGTTCTTTAGAAAATATTCAAAAACTTAGCGTCGAAGATAAGAGCTTCATAATGTTCC
AGAGGTAAACAACAACATAAATATTTTAAGATAAGTTGTTGGTCGTGGGATATGGTGACT
TATTTGTTTAACTCGAACTATTCATAAAGTTTAAATTTGATAAAACGTCAGGATTATATT
GAACTTTTTAAAAGAATTTAATCAAAACATTATAATTCTTCTAAAATTGAACTTTCTTTT
ATTTTTTCAAGCATTAAGAGATCACAACAGATAGAGTGTCAGAACAAATATATAATCAAA
CATAGCCTAACTGAATGGGAGAGAATGATAACAGGAATAAGGAACGACCAGAAGAAGTGA
ATAATGATATGATTACTTGGTGTACAAGACATCTTCCCACGGGTGTCGGTACAGTGGCTG
CTTCAGTCTCTTCTGATCGAATGTGTGCCTGCAACAATTTTATTCACGTAATCCACATCA
ATTTAAATTTGTTTTAAGAACAAGTGTAAGTGTTTGGTTATGACAGTTACCCGATTAAAC
CGATNGATCGAGCGA

Last Updated on 06.12.2018 16:11