Sequences from confirmation amplicon


Confirmation sequences for line 895H04 and hit At1g14120 are shown.
Line ID 895H04
Locus At1g14120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4834253
Primers used for PCR 63mk - 8474, show primer details
   
Sequence Name 35-K114217-0022-895-H04C-63mk
GenBank Accession LN501919 [GenBank]
Sequencing primer gene specific primer, 63mk: 5'-GCGTGATACTATAAGCACAACGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K114217-0022-895-H04C-63mk
TAAAATATGAGAATTAAAATATTTGAAGACAGATATATGTTGGTACATTTAATAGTCCGC
TTAATCTATCTTTGGGACGTCACTTAACTCATTTATGGTTATGAACTTGAGAGATTCGCC
AGCATGAAGATGCTTGCTAAGTCTAATCTTTCTTAGTCCTCCGTCACTGATAGGCTTGTA
TAGTCGCGGATGTTCAGCATCCACAAATTCATCTGGAGCTTCAAGATCTCTATCCACTGG
TCCTAATAGGAACGAAGCAATAGTAATCCTCATTTTTGCCTCTATGCATTGCACTCTATG
CTTCACATTGCATAACCTTCCATTGCTCCATATCTATCATAAAGTTTCAAAAACCAAAAA
AAATAAAGTTTAATTTTCATGTTCAACATATTTTTGGAACTATATACTAACTACGGGTGA
TATCATCCCACCGTAGCCATGTCACCAAGGTTGACAAGAAGCGTGTTCGGCAATGTGTGT
ATGGGGAAAAAGGATCCTGAAGAGTTGTCCATGGCTTCAAGTCCACCAACATCCTCATCA
CCTTGAAGAATTGTCAAGAAACCAGGATCCGTGTGTAATATCACACCGAGTTTTCCAACA
GAATCCGGTTTAAAATGATATTTGTTCATCCGAAATTGGCTTGGCCATCCTCTCANAAAA
TTGGGCCATTTACATGAAGCCATCANATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTC
   
Sequence Name 17-K114216-0022-895-H04C-8409
GenBank Accession LN493942 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K114216-0022-895-H04C-8409
CCTTTACATTGAATATGATGGCTTCTGTAAATGGCCCAATTTTTTGAGAGGATGGCCAAG
CCAATTTCGGATGAACAAATATCATTTTAAACCGGATTCTGTTGGAAAACTCGGTGTGAT
ATTACACACGGATCCTGGTTTCTTGACAATTCTTCAAGGTGATGAGGATGTTGGTGGACT
TGAAGCCATGGACAACTCTTCAGGATCCTTTTTCCCCATACACACATTGCCGAACACGCT
TCTTGTCAACCTTGGTGACATGGCTACGGTGGGATGATATCACCCGTAGTTAGTATATAG
TTCCAAAAATATGTTGAACATGAAAATTAAACTTTATTTTTTTTGGTTTTTGAAACTTTA
TGATAGATATGGAGCAATGGAAGGTTATGCAATGTGAAGCATAGAGTGCAATGCATAGAG
GCAAAAATGAGGATTACTATTGCTTCGTTCCTATNAGGACCAGTGGATAGAGATCTTGAA
GCTCCAGATGAATTTGTGGATGCTGAACATCCGCGACTATACAAGCCTATCAGTGACGGA
GGACTAAGAAAGATTAGACTTAGCAAGCATCTTCATGCTGGCGAATCTCTCAAGTTCATA
ACCATAAATGAGTTAAGTGACGTCCCAAAGATAGATTAAGCGGACTATTAAATGTACCAA
CATATATCTGTCTTCAAATATTTTAATTCTCATATTTTTATTTTTTGTTACCCTGAATAA
TGTAATAAATTTCGTTGTGC

Last Updated on 06.12.2018 16:11