Sequences from confirmation amplicon


Confirmation sequences for line 898G04 and hit At5g60730 are shown.
Line ID 898G04
Locus At5g60730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24424079
Primers used for PCR 21um - 8474, show primer details
   
Sequence Name 35-K117387-0022-898-G04C-21um
GenBank Accession LN501984 [GenBank]
Sequencing primer gene specific primer, 21um: 5'-ATGTTTTCAGGCATCAGCTAATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K117387-0022-898-G04C-21um
CTAAGCCCGTTCAGGTGTTGATTCTCCACTTCTTGCTCTTGAGGTGATTAAAAAACTACT
TGTCTCTGCAAACTCCGGGATACCTTACATATGTTGCTGAGTTTTCTTCTCATGTTGAAA
CAGATCACACCTGAGATAATGAAGGACGAGATAAAACGTCAGACGGGCGATAAAAGTGTG
AAAAATATGATGGATAGCATGGGGCTCGGAATGTTTGCCGGCGAGGTTCATTTTCCTTTT
CTCTCTTGTTGATTTTCCAAGCCTCCATTATGATTGGAAAACTTCATTTATGTCTTACCT
GTGTTGTTCATTTTTGGTAGTTAGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGC
   
Sequence Name 08-K117386-0022-898-G04C-8409
GenBank Accession LN489908 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K117386-0022-898-G04C-8409
AAATGACACACAGGTAAGACATAAATGAAGTTTTCCATCATAATGGAGGCTTGGAAAATC
AACAAGAGAGAAAAGGAAAATGAACCTCGCCGGCAAACATTCCGAGCCCCATGCTATCCA
TCATATTTTTCACACTTTTATCGCCCGTCTGACGTTTTATCTCGTCCTTCATTATCTCAG
GTGTGATCTGTTTCAACATGAGAAGAAAACTCAGCAACATATGTAAGGTATCCCGGAGTT
TGCAGAGACAAGTAGTTTTTTAATCACCTCAAGAGCAAGAAGTGGAGAATCAACACCTTG
AACGGGCTTTAGGACTCCTCCTGACAAGTCCTGGATTTCAAAACAAGAGATTAGCTGATG
CTTGNAAAACAT

Last Updated on 06.12.2018 16:11