Sequences from confirmation amplicon


Confirmation sequences for line 898H01 and hit At4g21323 are shown.
Line ID 898H01
Locus At4g21323 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11344944
Primers used for PCR 41pf - 8474, show primer details
   
Sequence Name 69-K115259-0022-898-H01C-41pf
GenBank Accession LN512261 [GenBank]
Sequencing primer gene specific primer, 41pf: 5'-GCTCAATGAAAATTAAGATAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K115259-0022-898-H01C-41pf
TGGCGAGTGTACAGTAAGTCGCAAGTCATCGGCAAGAGGGCCTAGTTCATTTTCACCTGC
CATTCTTAAGGTTTTGTTTCCTTCATCTCTTATTCCGGATCACTAAATCTATCATCTATG
TTTTATTCTTCAACTAACTTGTTTGTATTTTCTCTGTAATGCAGCCGGATATAGCAGCTC
CGGGTTTGACCTTGCTAACACCTAGAATACCAACAGACGAAGACACCAGAGAATTCGTTT
ACTCAGGAACATCAATGGCAACTCCGGTTATTGCAGGAATCGTAGCACTCCTCAAGATAT
CGCATCCTAATTGGTCTCCTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 29-K115258-0022-898-H01C-8409
GenBank Accession LN499417 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K115258-0022-898-H01C-8409
CATTTCATTGAATATATCCTGAGGAGACCAATTAGGATGCGATATCTTGAGGAGTGCTAC
GATTCCTGCAATAACCGGAGTTGCCATTGATGTTCCTGAGTAAACGAATTCTCTGGTGTC
TTCGTCTGTTGGTATTCTAGGTGTTAGCAAGGTCAAACCCGGAGCTGCTATATCCGGCTG
CATTACAGAGAAAATACAAACAAGTTAGTTGAAGAATAAAACATAGATGATAGATTTAGT
GATCCGGAATAAGAGATGAAGGAAACAAAACCTTAAGAATGGCAGGTGAAAATGAACTAG
GCCCTCTTGCCGATGACTTTGCGACTTTACTTGCTACACTTTCGCCAATTATTGTCTTGT
ATGGGCTTATCTTAATTTTCATTGAG

Last Updated on 06.12.2018 16:11