Sequences from confirmation amplicon


Confirmation sequences for line 900A10 and hit At2g17970 are shown.
Line ID 900A10
Locus At2g17970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7819230
Primers used for PCR 10ux - 8474, show primer details
   
Sequence Name 53-K117939-0022-900-A10C-10ux
GenBank Accession LN508289 [GenBank]
Sequencing primer gene specific primer, 10ux: 5'-AAAAACGGATATAAAAATACCCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K117939-0022-900-A10C-10ux
TGTTTGAAATTCAGACTTATGTATATATGTTGCACAAATTAATTATTTATATGGAAAATT
GGTTAATTAAATAAAGAACTTATAAATTTCAGTTTCAGAAGTTGAAGAATTTAACCGTAG
TTTCTTCTACTAGGACGTGGCATTCCTCTTCTCTGGCTTGATGGCCAGTCTCCAGAGTCA
TTGTGTTCACTGCTTCTCTCAGGATTATAGTAACCTCTTGAGTCAATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATA
ATATCCG
   
Sequence Name 44-K117938-0022-900-A10C-8409
GenBank Accession LN505327 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K117938-0022-900-A10C-8409
TTGAATTGACTCAGAGGTTACTATAATCCTGAGAGAAGCAGTGAACACAATGACTCTGGA
GACTGGCCATCAAGCCAGAGAAGAGGAATGCCACGTCCTAGTAGAAGAAACTACGGTTAA
ATTCTTCAACTTCTGAAACTGAAATTTATAAGTTCTTTATTTAATTAACCAATTTTCCAT
ATAAATAATTAATTTGTGCAACATATATACATAAGTCTTGAATTTTTTTTCAAACAATTA
TTTATGTCCGGGGTATTTTTATATCCGTTTTTAAAAAAGAAAGAGTGGTTTCTTTTTTTT
TTTCATTATGAAAAGGGGGGGTCCTCCTAATACAAAAAAA

Last Updated on 06.12.2018 16:11