Sequences from confirmation amplicon


Confirmation sequences for line 900D06 and hit At2g28420 are shown.
Line ID 900D06
Locus At2g28420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12158119
Primers used for PCR 33vz - 8474, show primer details
   
Sequence Name 22-K118392-0022-900-D06C-8409
GenBank Accession LN496313 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K118392-0022-900-D06C-8409
TTGAAGCCATGTGGCTTTTCAACTACGGCGTTGGGATCCACTTGGTGCAGGCCAAGGACC
AAGATAAGTTACCTTCTGACACGGACCATTTGGACCCAATGGATAACCACATCTCTTTCC
AGTGTGAAGATATGGAAGCTTTGGAGAAGAGGCTCAAGGAGGTGAAGGTAAAGTACATCA
AGAGGACGGTGGGTGATGAGAAAGATGCAGCTATCGACCAGCTCTTCTTCAATGACCCTG
ACGGCTTTATGGTTGAAATCTGTAACTGCGAGAATCTCGAACTTGTCCCGTGCCATTCAG
CCGACGCCATACGCCTCCCAGAAGACCGACATGCACCTCCTGTTGCCCTCCCTGACTCCT
CCAACCGTAGGATGCCCCAGCCCAATTCTTAATACTATTTAGACTCGTTTCGTCTCTAGT
TAGTACTGTGTTGTGCTCAGCTCCCATCCTATTAGTT
   
Sequence Name 70-K118393-0022-900-D06C-33vz
GenBank Accession LN512464 [GenBank]
Sequencing primer gene specific primer, 33vz: 5'-AACTAATAGAGTGGGAGCTGAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K118393-0022-900-D06C-33vz
GAATTGGGCTGGGGCATCCTACGGTTGGAGGAGTCAGGGAGGGCAACAGGAGGTGCATGT
CGGTCTTCTGGGAGGCGTATGGCGTCGGCTGAATGGCACGGGACAAGTTCGAGATTCTCG
CAGTTACAGATTTCAACCATAAAGCCGTCAGGGTCATTGAAGAAGAGCTGGTCGATAGCT
GCATCTTTCTCATCACCCACCGTCCTCTTGATGTACTTTACCTTCACCTCCTTGAGCCTC
TTCTCCAAAGCTTCCATATCTTCACACTGGAAAGAGATGTGGTTATCCATTGGGTCCAAA
TGGTCCGTGTCAGAAGGTAACTTATCTTGGTCCTTGGCCTGCACCAAGTGGATCCCAACG
CCGTAGTTGAAAAGCCACATGGCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11