Sequences from confirmation amplicon


Confirmation sequences for line 900H12 and hit At3g55130 are shown.
Line ID 900H12
Locus At3g55130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20436290
Primers used for PCR 43kk - 8474, show primer details
   
Sequence Name 75-K113845-0022-900-H12C-43kk
GenBank Accession LN513281 [GenBank]
Sequencing primer gene specific primer, 43kk: 5'-ACAATCGAGATTGCAAAACTTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K113845-0022-900-H12C-43kk
GCTTCCTCAGCACTCTCCCCGCTAGTGCATCGATCAACGTTGATTTTCCCGCTCCACTTG
CACCGAGAACGGCGAGGATATCGCCGTCAGAAGCCTCTCCGGAAACATCATCGAGTAGAG
TCTTTACTCCGTTTTGCCGTGAGAAGCCAAACCGCCGGCGAAGTGTGACATCGTATTGAA
GATTGTTAAAGTTTAAGACGTACGGGACAGGTTTTACGTACAAGGCTTCGACATCTATTA
TGTGATGTGCAGGATCGTCGCTTTTTACGCCGTCGCCGGAATCTCCTTTTCGGAAACTGT
CGCAGTCTTTCAGCAACTCGTCGAGGGTTGGTGATCCGATGGGAGTGATATACTGAGTTT
CCGCCGAAACACGTGTCATGGCAATCTTTCTACCGCTGAGTGATAGATTCATCGGACGGC
GTCGTTTAGAGTAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTTATT
   
Sequence Name 48-K113844-0022-900-H12C-8409
GenBank Accession LN506559 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K113844-0022-900-H12C-8409
GTCGATGATCTATCACTCAGCGGTAGAAAGATTGCCATGACACGTGTTTCGGCGGAAACT
CAGTATATCACTCCCATCGGATCACCAACCCTCGACGAGTTGCTGAAAGACTGCGACAGT
TTCCGAAAAGGAGATTCCGGCGACGGCGTAAAAAGCGACGATCCTGCACATCACATAATA
GATGTCGAAGCCTTGTACGTAAAACCTGTCCCGTACGTCTTAAACTTTAACAATCTTCAA
TACGATGTCACACTTCGCCGGCGGTTTGGCTTCTCACGGCAAAACGGAGTAAAGACTCTA
CTCGATGATGTTTCCGGAGAGGCTTCTGACGGCGATATCCTCGCCGTTCTCGGTGCAAGT
GGAGCAGGAAAATCAACGTTGATCGATGCACTAGCGGGGAGAGTTGCTGAGGGAAGCTTG
AGAGGCTCTGTAACTCTAAACGGAGAGAAAGTTTTGCATCATCGATTG

Last Updated on 06.12.2018 16:11