Sequences from confirmation amplicon


Confirmation sequences for line 901E02 and hit At1g61430 are shown.
Line ID 901E02
Locus At1g61430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22667170
Primers used for PCR DN48 - 8474, show primer details
   
Sequence Name 03-K101828-0022-901-E02C-8409
GenBank Accession LN487428 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K101828-0022-901-E02C-8409
ATTGAATAATCCTGCAACGCTTCATCTTTTACTCGACACGTTATTATAGAACCGGTCCAT
GGGCTAAAACAAGGTTCACTGGATCACCACAAATGGATGAATCATACACAAGTCCATTCA
TCTTAACACAGGATGTAAATGGGTCAGGATACTTTTCATTTGTTGAAAGAGGCAAACCTT
CACGCATGATTTTAACATCAGAGGGAACAATGAAGGTCTTAGTGCATAATGGAATGGACT
GGGAATCAACCTACGAGGGTCCAGCCAATTCATGTGATATATATGGCGTATGTGGACCTT
TTGGGCTTTGTGTTGTATCAATTCCTCCAAAATGTAAATGCTTCAAAGGTTTTGTTCCTA
AATTCGCTAAGGAGTGGAAGAAAGGAAACTGGACTATGGTTTGTGTGA
   
Sequence Name 47-K101831-0022-901-E02C-DN48
GenBank Accession LN506158 [GenBank]
Sequencing primer gene specific primer, DN48: 5'-TCACACAACCACTAGTCCAGTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K101831-0022-901-E02C-DN48
ACAAACCTTTGAGCATTTACATTTTGGAGGAATTGATACAACACAAAGCCCAAAAGGTCC
ACATACGCCATATATATCACATGAATTGGCTGGACCCTCGTAGGTTGATTCCCAGTCCAT
TCCATTATGCACTAAGACCTTCATTGTTCCCTCTGATGTTAAAATCATGCGTGAAGGTTT
GCCTCTTTCAACAAATGAAAAGTATCCTGACCCATTTACATCCTGTGTTAAGATGAATGG
ACTTGTGTATGATTCATCCATTTGTGGTGATCCAGTGAACCTTGTTTTAGCCCATGGACC
GGTTCTATAATAACGTGTCGAGTAAAAGATGAAGCGTTGCAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCAACGTTA
TTATAGAACCGGTCCATGGGCTAAAACAAGGTTCACTGGATCACCACAAATGNATGAATC
ATACACA
Primers used for PCR DN48 - 8474, show primer details
   
Sequence Name 60-K113261-0022-901-E02C-DN48
GenBank Accession LN510309 [GenBank]
Sequencing primer gene specific primer, DN48: 5'-TCACACAACCACTAGTCCAGTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K113261-0022-901-E02C-DN48
ACGATTAGGACAAACCTTTGAGCATTTACATTTTGGAGGAATTGATACAACACAAAGCCC
AAAAGGTCCACATACGCCATATATATCACATGAATTGGCTGGACCCTCGTAGGTTGATTC
CCAGTCCATTCCATTATGCACTAAGACCTTCATTGTTCCCTCTGATGTTAAAATCATGCG
TGAAGGTTTGCCTCTTTCAACAAATGAAAAGTATCCTGACCCATTTACATCCTGTGTTAA
GATGAATGGACTTGTGTATGATTCATCCATTTGTGGTGATCCAGTGAACCTTGTTTTAGC
CCATGGACCGGTTCTATAATAACGTGTCGAGTAAAAGATGAAGCGTTGCAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCAGCGTTATTAT
   
Sequence Name 41-K113260-0022-901-E02C-8409
GenBank Accession LN504147 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K113260-0022-901-E02C-8409
ATTTACAATTGAATATATCCTGCAACGCTTCATCTTTTACTCGACACGTTATTATAGAAC
CGGTCCATGGGCTAAAACAAGGTTCACTGGATCACCACAAATGGATGAATCATACACAAG
TCCATTCATCTTAACACAGGATGTAAATGGGTCAGGATACTTTTCATTTGTTGAAAGAGG
CAAACCTTCACGCATGATTTTAACATCAGAGGGAACAATGAAGGTCTTAGTGCATAATGG
AATGGACTGGGAATCAACCTACGAGGGTCCAGCCAATTCATGTGATATATATGGCGTATG
TGGACCTTTTGGGCTTTGTGTTGTATCAATTCCTCCAAAATGTAAATGCTTCAAAGGTTT
TGTTCCTAAATTCGCTAAGGAGTGGAAGAAAGGAAACTGGACTATGGTTGTGTGA

Last Updated on 06.12.2018 16:11