Sequences from confirmation amplicon


Confirmation sequences for line 901G06 and hit At1g66860 are shown.
Line ID 901G06
Locus At1g66860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24942279
Primers used for PCR BK16 - 8474, show primer details
   
Sequence Name 88-K104544-0022-901-G06C-BK16
GenBank Accession LN514864 [GenBank]
Sequencing primer gene specific primer, BK16: 5'-GTTAATCAACCGTGGTGTCCTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K104544-0022-901-G06C-BK16
AGCCAAGATTGAGTCCTTCGCGTTGTAAAACTATTTGGCCACTATACACTCTTTAAGTGG
CAAAACTTTTTTTAAAAAATTTATGTTCCTTTTTCAGCGGAGAGGATACTTTACCTGTGA
ACCTCTACAAATCCCTAAGTAAGGAATGTTTTGTTCAAGGCAGAGCTTTGCGAGGGCAAA
CTCAATAGAGTCTTTTTCTTTGTCGATAGCTGTATCACTTGCGTGTGTTTTCCGAATCTC
GTCGAGTTCTTGAGGTGAAAGGGACGATATTTCGGACTCGTAAAGAGAAGGGTCGATGTC
TTCTCCTTCACAGAGTAGAACTCCATGGATGGGCTTGAAGCTCTCTAGTAACTTGTGAAC
ACCAGCCACTCGTGGGACAATCACCGGTACAGCACCGTTCTCGACTATAAGATCAAGATG
GTACTCACCTAAAGCAAAACAATACAACATAATTAAATCTTTGAAAAATATAGATGAAAA
TGTGAGAACAAGAATATAACATTTTAAGGATGTTTCAAAATGTCTATTACCAACGAAATC
AACAAATTTGTTCTTGCGAAGAGTGCGTCTAGAGACAACAAGGACGCGAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CA
   
Sequence Name 94-K104545-0022-901-G06C-8409
GenBank Accession LN515309 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>94-K104545-0022-901-G06C-8409
CATTTAAATTGAATAATCCTCGCGTCCTTGTTGTCTCTAGACGCACTCTTCGCAAGAACA
AATTTGTTGATTTCGTTGGTAATAGACATTTTGAAACATCCTTAAAATGTTATATTCTTG
TTCTCACATTTTCATCTATATTTTTCAAAGATTTAATTATGTTGTATTGTTTTGCTTTAG
GTGAGTACCATCTTGATCTTATAGTCGAGAACGGTGCTGTACCGGTGATTGTCCCACGAG
TGGCTGGTGTTCACAAGTTACTAGAGAGCTTCAAGCCCATCCATGGAGTTCTACTCTGTG
AAGGAGAAGACATCGACCCTTCTCTTTACGAGTCCGAAATATCGTCCCTTTCACCTCAAG
AACTCGACGAGATTCGGAAAACACACGCAAGTGATACAGCTATCGACAAAGAAAAAGACT
CTATTGAGTTTGCCCTCGCAAAGCTCTGCCTTGAACAAAACATTCCTTACTTAGGGATTT
GTAGAGGTTCACAGGTAAAGTATCCTCTCCGCTGAAAAAGGAACATAAATTTTTTAAAAA
AAGTTTTGCCACTTAAAGAGTGTATAGTGGCCAAATAGTTTTACAACGCGAAGGACTCAA
TCTTTGGCTTTGACCAAGTGGGTGAGTTCTTAAATTACTAGGACACCAC

Last Updated on 06.12.2018 16:11