Sequences from confirmation amplicon


Confirmation sequences for line 901G09 and hit At1g61360 are shown.
Line ID 901G09
Locus At1g61360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22639138
Primers used for PCR KQ78 - 8474, show primer details
   
Sequence Name 29-K101500-0022-901-G09C-8409
GenBank Accession LN499222 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K101500-0022-901-G09C-8409
CATTTACATTGAATATATCCTGACCGAGGACATATATTTCCCGGATATCTATTTTGTCTT
CTCTATTCTGATATTTCTTGTTCTTTATATGACTGTCTGCAACTATGATGACTTCAGGGG
AAGCTTCAGGACGGAAAGGAAATAGCTGTAAAACGTCTTACTAGCAGCTCTGTACAGGGC
ACAGAGGAGTTCATGAACGAGATAAAACTCATCTCGAAATTACAACACAGAAACTTGCTT
CGGCTTTTGGGATGCTGCATCGATGGAGAAGAGAAGCTATTGGTCTATGAATATATGGTG
AACAAAAGCCTTGATATCTTTATATTTGGTAAGTCCCTTCCTGCTTTTCTCCTTCCATGA
CGATAGAGAGTTCTTTATGCTGATCGATTTCTGCCATACTCTGTGTGTTGTACTTTTGGC
CAGCAGATTTGAAGAAAAAGCTTGAGATTGATTGGGCTACGAGATTCAATATCATTCAAG
GAATCGCTCGTGGACTTCTCTATCTCAATCGTGA
   
Sequence Name 29-K101504-0022-901-G09C-KQ78
GenBank Accession LN499223 [GenBank]
Sequencing primer gene specific primer, KQ78: 5'-GTCACGATGGAGATAGAGAAGTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K101504-0022-901-G09C-KQ78
TGATCTCGTACCAATCAATCTCAAGCTTTTTCTTCAATCTGCTGGCCAAAAGTACAACAC
ACAGAGTATGGCAGAAATCGATCAGCATAAAGAACTCTCTATCGTCATGGAAGGAGAAAA
GCAGGAAGGGACTTACCAAATATAAAGATATCAAGGCTTTTGTTCACCATATATTCATAG
ACCAATAGCTTCTCTTCTCCATCGATGCAGCATCCCAAAAGCCGAAGCAAGTTTCTGTGT
TGTAATTTCGAGATGAGTTTTATCTCGTTCATGAACTCCTCTGTGCCCTGTACAGAGCTG
CTAGTAAGACGTTTTACAGCTATTTCCTTTCCGTCCTGAAGCTTCCCCTGAAGTCATCAT
AGTTGCAGACAGTCATATNAAGAACAAGAAATATCAGAATAGAGAAGACAAAATAGATNT
CCGGGAAATATATGTCCTCGGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCANAAATGTAGATTTCCGCA

Last Updated on 06.12.2018 16:11