Sequences from confirmation amplicon


Confirmation sequences for line 905D04 and hit At3g51590 are shown.
Line ID 905D04
Locus At3g51590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19136176
Primers used for PCR SD35 - 8474, show primer details
   
Sequence Name 56-K103548-0022-905-D04C-SD35
GenBank Accession LN509075 [GenBank]
Sequencing primer gene specific primer, SD35: 5'-CTGGCAAGTTTGATTAGTCGTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K103548-0022-905-D04C-SD35
ATATACTGCACATTCGAACAACCAAACAAATATCTATCATGAGTCATGACATTGGAAATA
ACTAAACTCCAAATGGTTTTATTCATACAAGCATTAAAAATGTACGTTAGTCCATTCTTC
TTATTACCCACAACATTTGAATAAGTTACAAAACGTTTGTTAAATACTCGATCATGTCAC
ATATTATATATTTTTATGTTTAAAAAGAGTTGTATTAGTGTTTGAATTTTTAATTAATAA
CTTCTAAAGTGTGTTTGTATAAATGTTTTAAAAATAAACGTAACAAGATAGTGACCAAAA
TTTTTAATATAAAATAAAATTGATATCTCCTAATTTACATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 40-K103549-0022-905-D04C-8409
GenBank Accession LN503682 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K103549-0022-905-D04C-8409
TTAGGAGATATCAATTTTATTTTATATTAAAAATTTTGGTCACTATCTTGTTACGTTTAT
TTTTAAAACATTTATACAAACACACTTTAGAAGTTATTAATTAAAAATTCAAACACTAAT
ACAACTCTTTTTAAACATAAAAATATATAATATGTGACATGATCGAGTATTTAACAAACG
TTTTGTAACTTATTCAAATGTTGTGGGTAATAAGAAGAATGGACTAACGTACATTTTTAA
TGCTTGTATGAATAAAACCATTTGGAGTTTAGTTATTTCCAATGTCATGACTCATGATAG
ATATTTGTTTTGGTTGTTCGAATGTTGCAGTATATCGACTGCCGTGTGAAAGAGGCTAGT
GATCAGATGTACGACTAATCAAACTTGCCA

Last Updated on 06.12.2018 16:11