Sequences from confirmation amplicon


Confirmation sequences for line 905D07 and hit At2g40780 are shown.
Line ID 905D07
Locus At2g40780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17022904
Primers used for PCR 82mm - 8474, show primer details
   
Sequence Name 63-K118635-0022-905-D07C-82mm
GenBank Accession LN511090 [GenBank]
Sequencing primer gene specific primer, 82mm: 5'-CAAAGATACTAGACCGATTCCAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K118635-0022-905-D07C-82mm
ACGGTGAAGTTGATTCGAGTGATGATGATGATGGTATGCCTCCATTGCAAGCAAACACAA
ACAGATTGAGACCGTTTGGGGTGAAGTGTGATGCAGAAACTGATTCAGGGTCAGATTCCG
ATTCATAGAAACATCCGGTACATTTCTTTTCGCAGCCTCAACTTAATTTCTCAATATAGG
GGATTTATAGTTGCAAGCTGTATTTTATAAACAGTATGTAACCAACACAGATCTGCTGAT
AAAGCAGAGTTTTGCTTTGCACTAAATCGATAATTGATTTATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 23-K118634-0022-905-D07C-8409
GenBank Accession LN496764 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K118634-0022-905-D07C-8409
ATTTACATTGAATATAAATCAATTATCGATTTAGTGCAAAGCAAAACTCTGCTTTATCAG
CAGATCTGTGTTGGTTACATACTGTTTATAAAATACAGCTTGCAACTATAAATCCCCTAT
ATTGAGAAATTAAGTTGAGGCTGCGAAAAGAAATGTACCGGATGTTTCTATGAATCGGAA
TCTGACCCTGAATCAGTTTCTGCATCACACTTCACCCCAAACGGTCTCAATCTGTTTGTG
TTTGCTTGCAATGGAGGCATACCATCATCATCATCACTCGAATCAACTTCACCGTCATCT
TCATGCTGTTCAATGGGTGAGCTTTTCTCAGCTGGAATCGGTCTAGTTATCTTTG

Last Updated on 06.12.2018 16:11