Sequences from confirmation amplicon


Confirmation sequences for line 907F01 and hit At3g28490 are shown.
Line ID 907F01
Locus At3g28490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10680045
Primers used for PCR 76we - 8474, show primer details
   
Sequence Name 85-K118393-0022-907-F01C-76we
GenBank Accession LN514588 [GenBank]
Sequencing primer gene specific primer, 76we: 5'-GAAAGTGAATTTCGTGGTTTCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K118393-0022-907-F01C-76we
CAAACATGTTATTAAGTTTTCCTCGTTTTCATAAAATTATATAGATTTTGTGATCATATA
GGACTGTGTTGTGGATGTAAACATTGGTTTTAGGCAAAAGGAAAGCTTGAGAAGTCAATG
GTGGTGGCTGATGTTGATTCAGGTGAGAGTGAAGACAGTGAAGTACGCACAAGTTCTGGA
ATGTTTCTGACCAAAAGACAGGTGAGAGCAGTGGAATTTTACTATGTATTTTTATTGTAT
TCGACTAGCTTAATTCACTAAATAGACAGACTAGTTCTCTGTGTTTTTTTTTGTAAAGAA
GTAAACGCTTGAGTCTTGACCAAGTTACTATTGGTTTTGTTTTACCAAGGTCCAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCC
   
Sequence Name 37-K118392-0022-907-F01C-8409
GenBank Accession LN502782 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K118392-0022-907-F01C-8409
TTGAAATATCCTGGACCTTGGTAAAACAAAACCAATAGTAACTTGGTCAAGACTCAAGCG
TTTACTTCTTTACAAAAAAAAACACAGAGAACTAGTCTGTCTATTTAGTGAATTAAGCTA
GTCGAATACAATAAAAATACATAGTAAAATTCCACTGCTCTCACCTGTCTTTTGGTCAGA
AACATTCCAGAACTTGTGCGTACTTCACTGTCTTCACTCTCACCTGAATCAACATCAGCC
ACCACCATTGACTTCTCAAGCTTTCCTTTTGCCTAAAACCAATGTTTACATCCACAACAC
AGTCCTATATGATCACAAAATCTATATAATTTTATGAAAACAAGAAAACTTAATAACATG
TTTGATTCTATACCAACTTAATCAAATGATCACATTCCTCATCTGAGAGAAATCCTTTGT
ATAAAAATGCCCTGAAGAAACCAC

Last Updated on 06.12.2018 16:11