Sequences from confirmation amplicon


Confirmation sequences for line 909C05 and hit At5g09800 are shown.
Line ID 909C05
Locus At5g09800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3043534
Primers used for PCR 19cc - 8474, show primer details
   
Sequence Name 56-K108225-0022-909-C05C-19cc
GenBank Accession LN509124 [GenBank]
Sequencing primer gene specific primer, 19cc: 5'-ACACCTTAAGAAGATCCGTTAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K108225-0022-909-C05C-19cc
TCTGTAAAGTACAGTATCTTCGTAACGCCGTTAATTCTAATAACGGCATCTTGCGCCTTC
TTTTCCTTGAAAAGATAACAGACGCTCCACAAAACCGTAACGGCGTGTTCAGTCGCCGCC
GTCGACACTTTCATCAGCTTATTCACCACCGTCTCCACGCACACACCATCACAAATCTCC
GATCGACCTTCTTTACAAGACGAAATAGCTTCGAGAAGCTTTAAGCATTTTTCCGTCACT
GAAACGCTCGTAGTTGGATCTGTTAACAAACTCGTGAGTTTCGTGACTAGCAATGAGTAA
AAGAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAC
GNTTATT
   
Sequence Name 40-K108224-0022-909-C05C-8409
GenBank Accession LN503730 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K108224-0022-909-C05C-8409
TTCTTTTCTCATTGCTAGTCCGAAACTCACGAGTTTGTTAACAGATCCAACTACGAGCGT
TTCAGTGACGGAAAAATGCTTAAAGCTTCTCGAAGCTATTTCGTCTTGTAAAGAAGGTCG
ATCGGAGATTTGTGATGGTGTGTGCGTGGAGACGGTGGTGAATAAGCTGATGAAAGTGTC
GACGGCGGCGACTGAACACGCCGTTACGGTTTTGTGGAGCGTCTGTTATCTTTTCAAGGA
AAAGAAGGCGCAAGATGCCGTTATTAGAATTAACGGCGTTACGAAGATACTGTTACTTTT
ACAGAGTAATTGCTCGCTTACCGTTAGGCATATGCTAACGGATCTTCTTAAGGTG

Last Updated on 06.12.2018 16:11