Sequences from confirmation amplicon


Confirmation sequences for line 911B06 and hit At2g18600 are shown.
Line ID 911B06
Locus At2g18600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8074089
Primers used for PCR 21vy - 8474, show primer details
   
Sequence Name 53-K118278-0022-911-B06C-21vy
GenBank Accession LN508293 [GenBank]
Sequencing primer gene specific primer, 21vy: 5'-GTGAAGAGATGGAAAAGACCGTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K118278-0022-911-B06C-21vy
CGGTTTCAGTCTTCCGCAAGATGTTCGACAGACGTTTCCTTCTAGATCGATATTCGGATG
ATACACCTACAACATTGACATTCTTTAGCATTCTGTATAAACATATCAGACAACATAAAA
CGCTTCAGAGACTGAGGAAAAAAAGCCTAACCTTGGTCTTGCATTTCACTTTAGGAGCTT
CGTGGGGGTACATATTAGAGACTTGGAATGAGAACACAAAGTTACCACTCCTGTTTAGGT
ATAACAAGAACGAATGAGTGTGTTTTTGCGCAAATTCAGGGACCCAAAAACAAAAATGAA
AGATAGAGATTTTGTTTACTTACAAGTAATATCCTTCATCAGGTTTAATGGTTACTTCAA
AATTCATCAAGTCATTCTTCCCATTCGGAAAAGATATCTTACAAGACTTTGGTAAGTTCA
GCTCCGATATATCTATGCACAAATAAACAGAGATGATTTATCAGTTACACAAAATCAATA
CTTGATATATTCAAATAGAACCCTAAAAATACTTGATATATTCATCAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATGTCCGCA
   
Sequence Name 21-K118277-0022-911-B06C-8409
GenBank Accession LN495857 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K118277-0022-911-B06C-8409
TCATTGAATTATCCTGATGAATATATCAAGTATTTTTAGGGTTCTATTTGAATATATCAA
GTATTGATTTTGTGTAACTGATAAATCATCTCTGTTTATTTGTGCATAGATATATCGGAG
CTGAACTTACCAAAGTCTTGTAAGATATCTTTTCCGAATGGGAAGAATGACTTGATGAAT
TTTGAAGTAACCATTAAACCTGATGAAGGATATTACTTGTAAGTAAACAAAATCTCTATC
TTTCATTTTTGTTTTTGGGTCCCTGAATTTGCGCAAAAACACACTCATTCGTTCTTGTTA
TACCTAAACAGGAGTGGTAACTTTGTGTTCTCATTCCAAGTCTCTAATATGTACCCCCAC
GAAGCTCCTAAAGTGAAATGCAAGACCAAGGTTAGGCTTTTTTTCCTCAGTCTCTGAAGC
GTTTTATGTTGTCTGATATGTTTATACAGAATGCTAAAGAATGTCAATGTTGTAGGTGTA
TCATCCGAATATCGATCTAGAAGGAAACGTCTGTCTGAACATCTTGCGTGAAGACTGGAA
ACCGGTTCTCAACATAAACACAGTTATCTACGGTCTT

Last Updated on 06.12.2018 16:11