Sequences from confirmation amplicon


Confirmation sequences for line 911G06 and hit At4g20930 are shown.
Line ID 911G06
Locus At4g20930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11199465
Primers used for PCR 11ck - 8474, show primer details
   
Sequence Name 56-K108442-0022-911-G06C-11ck
GenBank Accession LN509125 [GenBank]
Sequencing primer gene specific primer, 11ck: 5'-CAAGTCGAGATTGTTTCATTTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K108442-0022-911-G06C-11ck
ATATTGGGAAAGCCAGTCATGTTGGATGCTCCCGTCTCTGGAGGTGTTCTTGCTGCAGAA
GCTGGTACACTTACTTTCATGGTATGTTGACGACTCTCTTATCTTCTACATTATTTGAAT
CACATCCTTTATATAACATCAAGTTTGAAGATTATGTGTGCTTGTATACCTACAAGAGCA
CAATGGCATTGATACAACTGCACGTTCTGTAGGTTGGAGGTCCCGAAGATGCTTACTTAG
CCGCTAGACCAATACTTCAGTCAATGGGCAGAACCTCCATCTACTGTGGTGGTTCAGGAA
ATGGATCTGTAAGTTCGCTTTATACCATGTTTCCGTAACTCTGTTACAGTATCTCTATTA
TCTTGATCTACATGGCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCACAACGTTATT
   
Sequence Name 44-K108441-0022-911-G06C-8409
GenBank Accession LN505208 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K108441-0022-911-G06C-8409
GCCATTTACAATTGAAGCCATGTAGATCAAGATAATAGAGATACTGTAACAGAGTTACGG
AAACATGGTATAAAGCGAACTTACAGATCCATTTCCTGAACCACCACAGTAGATGGAGGT
TCTGCCCATTGACTGAAGTATTGGTCTAGCGGCTAAGTAAGCATCTTCGGGACCTCCAAC
CTACAGAACGTGCAGTTGTATCAATGCCATTGTGCTCTTGTAGGTATACAAGCACACATA
ATCTTCAAACTTGATGTTATATAAAGGATGTGATTCAAATAATGTAGAAGATAAGAGAGT
CGTCAACATACCATGAAAGTAAGTGTACCAGCTTCTGCAGCAAGAACACCTCCAGAGACG
GGAGCATCCAACATGACTGGCTTTTCCCAATTATCTACATCAAAGATGCTTGAAACTAGT
GAAAATGAAACAATCTCGACTTG

Last Updated on 06.12.2018 16:11