Sequences from confirmation amplicon


Confirmation sequences for line 912E07 and hit At1g34060 are shown.
Line ID 912E07
Locus At1g34060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12396957
Primers used for PCR BU31 - 8474, show primer details
   
Sequence Name 16-K104352-0022-912-E07C-BU31
GenBank Accession LN493415 [GenBank]
Sequencing primer gene specific primer, BU31: 5'-TAATCCAGATGGGAAATTGAAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K104352-0022-912-E07C-BU31
CACTTCTGATTATGCTTATTACTGGCCTCATTTCTCACCTATTACACATCCAGTTGATGA
AGATTTGAGCCTGTTTAGTCTCTCCAAGACTACAGGTCATGCTGGTTCAAGATTCGGGTG
ACTGATTTACTTTTACCCTCCTAGTACTCTAAAAACAATGTTCTGGTTTGGTCACAACAG
CTCACAAGTTTTTCTTGATGCAGGTGGGGATTGGTAAAGGACAAAGCTATTTATGAAAAA
ATGGATAGATTTATACGTTTAACTAGTATGGGAGTTTCTAAAGAAACACAGCTTCATGTT
CTTCAGTTGCTCAAAGTAGTAGTTGGTGATGGAGGCAATGAGATATTCAGTTTTGGTTAT
GGAACTGTGAAGAAAAGATGGGAGACACTGAACAAGATCTTCTCCATGCAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 32-K104353-0022-912-E07C-8409
GenBank Accession LN500568 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K104353-0022-912-E07C-8409
GAGAAGATCTTGTTCAGTGTCTCCCATCTTTTCTTCACAGTTCCATAACCAAAACTGAAT
ATCTCATTGCCTCCATCACCAACTACTACTTTGAGCAACTGAAGAACATGAAGCTGTGTT
TCTTTAGAAACTCCCATACTAGTTAAACGTATAAATCTATCCATTTTTTCATAAATAGCT
TTGTCCTTTACCAATCCCCACCTGCATCAAGAAAAACTTGTGAGCTGTTGTGACCAAACC
AGAACATTGTTTTTAGAGTACTAGGAGGGTAAAAGTAAATCAGTCACCCGAATCTTGAAC
CAGCATGACCTGTAGTCTTGGAGAGACTAAACAGGCTCAAATCTTCATCAACTGGATGTG
TAATAGGTGAGAAATGAGGCCAGTAATAAGCATAATCATGAAGTGTTTTCACATTAGGAC
CATCAAGAACCGCTCTTTTCAATTTCCCATCTGGAT

Last Updated on 06.12.2018 16:11