Sequences from confirmation amplicon


Confirmation sequences for line 913A02 and hit At1g71180 are shown.
Line ID 913A02
Locus At1g71180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26832891
Primers used for PCR SD16 - 8474, show primer details
   
Sequence Name 90-K103502-0022-913-A02C-SD16
GenBank Accession LN515037 [GenBank]
Sequencing primer gene specific primer, SD16: 5'-GAAACTCATCGATCCTTCAAAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K103502-0022-913-A02C-SD16
ATCGGGATAATGGGATCGGCCATGGTTTCTCATATTATCGCCGCAGGCTATTCTGTCACA
GTTTACGCTCGCGACCTCCGGAAAACAAAAGATCTGCAAACCAAAGGAGCTCGCATAGCC
AATAGCCCCAAAGAGTTAGCGGAGATGAGCGACGTTGTGTTCACGATTGTTGGAAACTTC
AACGATGTCCGATCATTGCTTCTCGGTGACGATGGTGTACTCTCTGGTCTTACGCCCGGT
GGAGTAACAGTGGACATGACTAGTAGCAAACCAGGATTGGCGCGTGAAATACACGCAGAA
GCGAGGCGTAGGAATTGTTGGGCGGTGGATGCGCCGGTATCAGGTGGAGACGCTGGAGCA
CGAGAGGGGACGTTAGGGATTTTCGCCGGCGGAGATTCAGAGATTGTGGAGTGGTTGTCC
CCGGTGATGAAGAAATCACCAGAACCATACATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATATCCGCACGGTTATTATAT
   
Sequence Name 93-K103503-0022-913-A02C-8409
GenBank Accession LN515250 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K103503-0022-913-A02C-8409
GCCATGTATGGTTCTGGTGATTTCTTCATCACCGGGGACAACCACTCCACAATCTCTGAA
TCTCCGCCGGCGAAAATCCCTAACGTCCCCTCTCGTGCTCCAGCGTCTCCACCTGATACC
GGCGCATCCACCGCCCAACAATTCCTACGCCTCGCTTCTGCGTGTATTTCACGCGCCAAT
CCTGGTTTGCTACTAGTCATGTCCACTGTTACTCCACCGGGCGTAAGACCAGAGAGTACA
CCATCGTCACCGAGAAGCAATGATCGGACATCGTTGAAGTTTCCAACAATCGTGAACACA
ACGTCGCTCATCTCCGCTAACTCTTTGGGGCTATTGGCTATGCGAGCTCCTTTGGTTTGC
AGATCTTTTGTTTTCCGGAGGTCGCGAGCGTAAACTGTGACAGAATAGCCTGCGGCGATA
ATATGAGAAACCATGGCCGATCCCATTATCCCGATGCCAATCCATCCTATTCGAGTCTTT
GAAGGATCATTGAGTTTC

Last Updated on 06.12.2018 16:11