Sequences from confirmation amplicon


Confirmation sequences for line 913A03 and hit At2g45660 are shown.
Line ID 913A03
Locus At2g45660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18809035
Primers used for PCR BC17 - 8474, show primer details
   
Sequence Name 45-K103981-0022-913-A03C-BC17
GenBank Accession LN505510 [GenBank]
Sequencing primer gene specific primer, BC17: 5'-GCTCTTTCGTAGCCAATTTACACT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K103981-0022-913-A03C-BC17
TGATCCATAGTATTAATAAACAACTTTCAATTAGTATTCTTTTGGTTTGAACTAATCTTT
GTCTTGTTATTGCTTTAAGCAAAACATGTTGTTCTAATTTCTAAGTGATGATTAGGAAGT
TGTTTCATCATTCCTGATTTATTAATCCCTCATGCTTCATTTCATGCTCATTCCTAATTT
AGTTCAATTTGTTTGAATATTTGTTCCTGATTTTGACATAGAAACTCAAAGCTAGCTAGC
CAAACCTAAATGTTGATTGTTTTTGAGAATCAAAAGAGTTTTATCTTGTACTGTTAGGTA
GTAGGGAAACCAAACTTACTTTTGATGAATCATTACTTCTGTAAATGAAAATGCCAGCTT
TTGATCAGATGTTTCAGACATTTGGTCCATTTGGGAAAGTACTTCTTTCTCTCGAACCTA
CTAAATATAAAGATAAGACCTCACATGTTTTTGATTTTCTAAAATAGGGGGAAAAAGTAC
AAGACTTTTCAAGCTATGTCCTTGATTAAGTCTAGTGATATCTTCAATAAGAAATGTTTT
GAGAACACCATTGGGATCTAAATTTGATCTCTGATGATTTACTTTAATGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 61-K103982-0022-913-A03C-8409
GenBank Accession LN510505 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K103982-0022-913-A03C-8409
GCCATTTCATTAAAGTAAATCATCAGAGATCAAATTTAGATCCCAATGTGTGTTCTCAAA
ACATTTCTTATTGAAGATATCACTAGACTTAATCAAGGACATAGCTTGAAAAGTCTTGTA
CTTTTTCCCCCTATTTTAGAAAATCAAAAACATGTGAGGTCTTATCTTTATATTTAGTAG
GTTCGAGAGAAAGAAGTACTTTCCCAAATGGACCAAATGTCTGAAACATCTGATCAAAAG
CTGGCATTTTCATTTACAGAAGTAATGATTCATCAAAAGTAAGTTTGGTTTCCCTACTAC
CTAACAGTACAAGATAAAACTCTTTTGATTCTCAAAAACAATCAACATTTAGGTTTGGCT
AGCTAGCTTTGAGTTTCTATGTCAAAATCAGGAACAAATATTCAAACAAATTGAACTAAA
TTAGGAATGAGCATGAAATGAAGCATGAGGGATTAATAAATCAGGAATGATGAAACAACT
TCCTAATCATCACTTAGAAATTAGAACAACATGTTTTGCTTAAAGCAATAACAAGACAAA
GATTAGTTCAAACCAAAAGAATACTAATTGAAAGTTGTTTTATTAATACTATGTGATCAT
GATCTCTAAGAAGATCAAATATATAACAAGTGTAAATT

Last Updated on 06.12.2018 16:11