Sequences from confirmation amplicon


Confirmation sequences for line 913B02 and hit At5g46750 are shown.
Line ID 913B02
Locus At5g46750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18970209
Primers used for PCR 27gz - 8474, show primer details
   
Sequence Name 37-K112477-0022-913-B02C-27gz
GenBank Accession LN502666 [GenBank]
Sequencing primer gene specific primer, 27gz: 5'-TTTGCCTCTCGATTTGAGTACTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K112477-0022-913-B02C-27gz
ACACGGGGGCTTGCCATGTTGCTCCACCAAAGTCATCAAATTTCTTTAATGAATTTGGAA
TGGACAGTGCTTTCCCCAAGAAGTCAAGCTCAAGCTCATCCAAAGCTCAGGTAAAGAAAA
GTTTCATTTTTAACAGAACTGAAGAAAGAGATAGAAATCAATTCTCGATTCCTCTTTGTT
TACCTGTTTTCTTTTTACTTAGGTTGAAGAAACAGATGAAGCAAGAAAGAAGTTTTCAAA
CGCCAAATCGATTTCCTCTGCCCAATTTTTCGGAAATCAGAACAGAGATGCCGATCTTGA
CTCAAAAGCTACCCTTCAGAAGTTCTCGGTATGTCCTTAACCGATCAAACTCTATACTAG
TTTTCTTTAGTCTCGTTGTTCATACATGCCTAATGCGATTACAAATCTATCAGGGTTCAG
CAGCTATTTCAAGTTCTGATCTTTTTGGCCACGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 14-K112476-0022-913-B02C-8409
GenBank Accession LN492573 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K112476-0022-913-B02C-8409
TTTCATTGAATTATCCGTGGCCAAAAGATCAGAACTTGAAATAGCTGCTGAACCCTGATA
GATTTGTAATCGCATTAGGCATGTATGAACAACGAGACTAAAGAAAACTAGTATAGAGTT
TGATCGGTTAAGGACATACCGAGAACTTCTGAAGGGTAGCTTTTGAGTCAAGATCGGCAT
CTCTGTTCTGATTTCCGAAAAATTGGGCAGAGGAAATCGATTTGGCGTTTGAAAACTTCT
TTCTTGCTTCATCTGTTTCTTCAACCTAAGTAAAAAGAAAACAGGTAAACAAAGAGGAAT
CGAGAATTGATTTCTATCTCTTTCTTCAGTTCTGTTAAAAATGAAACTTTTCTTTACCTG
AGCTTTGGATGAGCTTGAGCTTGACTTCTTGGGGAAAGCACTGTCCATTCCAAATTCATT
AAAGAAATTTGATGACTTTGGTGGAGCAACATGGCTAAGCACCCGTGTGCCACTTTGCCC
ACCAGATTGCTCATCATCAAAGTACTCAAATCAGNAGGCAA

Last Updated on 06.12.2018 16:11