Sequences from confirmation amplicon


Confirmation sequences for line 913H04 and hit At5g05260 are shown.
Line ID 913H04
Locus At5g05260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1561531
Primers used for PCR NC50 - 8474, show primer details
   
Sequence Name 73-K101328-0022-913-H04C-8409
GenBank Accession LN512861 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K101328-0022-913-H04C-8409
TCGGACTGAGCCTTTACAATTGAATATATCCTGAAATACTCACGTGATCCCCGTGACATC
CCCGAGAATTGCAAGAGAGATTCTGAAGAAGCAAGACTCCGTTTTCGCCACTAGACCGCT
AACGATGGGCACGGAGTACTGCAGCCGCGGGTACTTGACCGTTGCGGTGGAGCCACAAGG
AGAGCAGTGGAAGAAGATGAGGAGAGTGGTGGCATCTCACGTGACGAGCAAGAAGAGCTT
CCAAATGATGCTACAAAAGAGAACCGAAGAGGCTGATAACTTAGTCCGGTACATCAATAA
CCGTAGTGTCAAAAACCGTGGTAATGCTTTTGTGGTTATTGATTTAAGGCTTGCGGTACG
GCAATACAGTGGAAATGTAGCTCGGAAGATGATGTTTGGTATAAGGCATTTTGGTAAAGG
AAGTGAAGATGGATCGGGACCAGGGTTGGAAGAGATTGAACATGTGGAATCTTTGTTTAC
GGTTTTAAC
   
Sequence Name 73-K101334-0022-913-H04C-NC50
GenBank Accession LN512862 [GenBank]
Sequencing primer gene specific primer, NC50: 5'-CGTAAAGATGGGTTAAAACCGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K101334-0022-913-H04C-NC50
TCTCTACACCCTGGTCCCGATCCATCTTCACTTCCTTTACCAAAATGCCTTATACCAAAC
ATCATCTTCCGAGCTACATTTCCACTGTATTGCCGTACCGCAAGCCTTAAATCAATAACC
ACAAAAGCATTACCACGGTTTTTGACACTACGGTTATTGATGTACCGGACTAAGTTATCA
GCCTCTTCGGTTCTCTTTTGTAGCATCATTTGGAAGCTCTTCTTGCTCGTCACGTGAGAT
GCCACCACTCTCCTCATCTTCTTCCACTGCTCTCCTTGTGGCTCCACCGCAACGGTCAAG
TACCCGCGGCTGCAGTACTCCGTGCCCATCGTTAGCGGTCTAGTGGCGAAAACGGAGTCT
TGCTTCTTCAGAATCTCTCTTGCAATTCTCGGGGATGTCACGGGGATCACGTGAGTATTT
CAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11